Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy

被引：80

作者：

Hunter, Jennifer J. ^{[1
,3
]}

Masella, Benjamin ^{[2
,3
]}

Dubra, Alfredo ^{[1
,3
]}

Sharma, Robin ^{[2
,3
]}

Yin, Lu ^{[3
]}

Merigan, William H. ^{[1
,3
]}

Palczewska, Grazyna ^{[4
]}

Palczewski, Krzysztof ^{[4
,5
]}

Williams, David R. ^{[1
,2
,3
]}

机构：

[1] Univ Rochester, Flaum Eye Inst, Rochester, NY 14642 USA

[2] Univ Rochester, Inst Opt, Rochester, NY 14642 USA

[3] Univ Rochester, Ctr Visual Sci, Rochester, NY 14642 USA

[4] Polgenix Inc, Cleveland, OH 44106 USA

[5] Case Western Reserve Univ, Dept Pharmacol, Cleveland, OH 44106 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2011年 / 2卷 / 01期

关键词：

RETINAL-PIGMENT EPITHELIUM; HUMAN CONE PHOTORECEPTORS; IN-VIVO FLUORESCENCE; INTRINSIC SIGNALS; OUTER SEGMENTS; CELLS; MICROSCOPY; ORIGINS; LIGHT; CYCLE;

D O I：

10.1364/BOE.2.000139

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In vivo two-photon imaging through the pupil of the primate eye has the potential to become a useful tool for functional imaging of the retina. Two-photon excited fluorescence images of the macaque cone mosaic were obtained using a fluorescence adaptive optics scanning laser ophthalmoscope, overcoming the challenges of a low numerical aperture, imperfect optics of the eye, high required light levels, and eye motion. Although the specific fluorophores are as yet unknown, strong in vivo intrinsic fluorescence allowed images of the cone mosaic. Imaging intact ex vivo retina revealed that the strongest two-photon excited fluorescence signal comes from the cone inner segments. The fluorescence response increased following light stimulation, which could provide a functional measure of the effects of light on photoreceptors. (C)2010 Optical Society of America

引用

页码：139 / 148

页数：10

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