Rational design of boron dipyrromethene (BODIPY)-based photobleaching-resistant fluorophores applicable to a protein dynamics study

被引：55

作者：

Komatsu, Toru ^{[1
]}

Oushiki, Daihi ^{[1
]}

Takeda, Aoi ^{[1
]}

Miyamura, Masaki ^{[3
]}

Ueno, Tasuku ^{[1
]}

Terai, Takuya ^{[1
]}

Hanaoka, Kenjiro ^{[1
]}

Urano, Yasuteru ^{[2
]}

Mineno, Tomoko ^{[3
]}

Nagano, Tetsuo ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] Univ Tokyo, Sch Med, Tokyo 1130033, Japan

[3] Takasaki Univ Hlth & Welf, Fac Pharm, Takasaki, Gunma 3700033, Japan

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 36期

关键词：

FLUORESCENCE PROBES; SMALL MOLECULES; BODIPY DYES;

D O I：

10.1039/c1cc13367e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We studied the photobleaching of a library of boron dipyrromethene (BODIPY) derivatives with a range of electron densities, and found that the photobleaching rate is influenced by the electron-withdrawing capacity of the substituents. Electron-deficient BODIPYs generated less singlet oxygen, were less reactive to singlet oxygen, and were highly resistant to photobleaching. We confirmed the utility of one of these fluorophores, 2,6-diCO(2)R-BDP, for visualizing EGF receptor dynamics in cells expressing an SNAP-tagged EGF receptor.

引用

页码：10055 / 10057

页数：3

共 24 条

[1] Highly Substituted Bodipy Dyes with Spectroscopic Features Sensitive to the Environment [J].