Light sheet fluorescence microscopy using high-speed structured and pivoting illumination

被引：13
|
作者：
Itoh, Ryosuke [1 ]
Landry, Joseph Russell [2 ]
Hamann, Stephen Sanborn [2 ]
Solgaard, Olav [2 ]
机构：
[1] SCREEN Holdings Co Ltd, R&D Dept, Fushimi Ku, 322 Furukawa Cho, Kyoto 6128486, Japan
[2] Stanford Univ, Edward L Ginzton Lab, Stanford, CA 94305 USA
来源：
OPTICS LETTERS | 2016年 / 41卷 / 21期
关键词：
D O I：
10.1364/OL.41.005015
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We show that light sheet fluorescence microscopy with structured and pivoting illumination enables fast image acquisition and improved image quality. A one-dimensional spatial light phase modulator is used to control the illumination profile at high speed. To demonstrate the features of the system, we image fluorescent beads and biological samples, successfully obtaining optically sectioned images with higher contrast using structured illumination and with reduced shadowing effects using pivoting illumination. (C) 2016 Optical Society of America
引用
收藏
页码：5015 / 5018
页数：4
相关论文
共 50 条
  • [1] Advances in High-Speed Structured Illumination Microscopy
    Zhao, Tianyu
    Wang, Zhaojun
    Chen, Tongsheng
    Lei, Ming
    Yao, Baoli
    Bianco, Piero R.
    FRONTIERS IN PHYSICS, 2021, 9
  • [2] High-Speed Structured Illumination Microscopy and Its Applications
    Zhao Tianyu
    Wang Zhaojun
    Feng Kun
    Liang Yansheng
    He Minru
    Yun Xue
    Lei Ming
    LASER & OPTOELECTRONICS PROGRESS, 2020, 57 (24)
  • [3] Characterization of neurite dystrophy after trauma by high speed structured illumination microscopy and lattice light sheet microscopy
    Phillips, Jack K.
    Sherman, Sydney A.
    Cotton, Kristen Y.
    Heddleston, John M.
    Taylor, Aaron B.
    Finan, John D.
    JOURNAL OF NEUROSCIENCE METHODS, 2019, 312 : 154 - 161
  • [4] High-speed image reconstruction for nonlinear structured illumination microscopy
    Zhang, Jingxiang
    Zhao, Tianyu
    Fu, Xiangda
    Shu, Manming
    Yan, Jiajing
    Wang, Mengrui
    Liang, Yansheng
    Wang, Shaowei
    Lei, Ming
    PHOTONICS RESEARCH, 2025, 13 (03) : 743 - 760
  • [5] High-speed image reconstruction for nonlinear structured illumination microscopy
    JINGXIANG ZHANG
    TIANYU ZHAO
    XIANGDA FU
    MANMING SHU
    JIAJING YAN
    MENGRUI WANG
    YANSHENG LIANG
    SHAOWEI WANG
    MING LEI
    Photonics Research, 2025, 13 (03) : 743 - 760
  • [6] High-speed spatially re-modulated structured illumination microscopy
    Tu, Shijie
    Li, Xiaoyan
    Wang, Yueying
    Gong, Wenwen
    Liu, Xin
    Liu, Q.
    Han, Yubing
    Kuang, Cuifang
    Liu, Xu
    Hao, Xiang
    OPTICS LETTERS, 2023, 48 (10) : 2535 - 2538
  • [7] High-Speed Imaging of Amoeboid Movements Using Light-Sheet Microscopy
    Takao, Daisuke
    Taniguchi, Atsushi
    Takeda, Takaaki
    Sonobe, Seiji
    Nonaka, Shigenori
    PLOS ONE, 2012, 7 (12):
  • [8] Multiplane Illumination Enabled by Fourier-Transform Metasurfaces for High-Speed Light-Sheet Microscopy
    Shi, Fenghua
    Qiu, Meng
    Zhang, Lei
    Lam, Edmund Y.
    Lei, Dang Yuan
    ACS PHOTONICS, 2018, 5 (05): : 1676 - 1684
  • [9] High-Speed Large-Field Multifocal Illumination Fluorescence Microscopy
    Chen, Zhenyue
    Mc Larney, Benedict
    Rebling, Johannes
    Dean-Ben, Xose Luis
    Zhou, Quanyu
    Gottschalk, Sven
    Razansky, Daniel
    LASER & PHOTONICS REVIEWS, 2020, 14 (02)
  • [10] High-speed multicolor structured illumination microscopy using a hexagonal single mode fiber array
    Hinsdale, Taylor A.
    Stallinga, Sjoerd
    Rieger, Bernd
    BIOMEDICAL OPTICS EXPRESS, 2021, 12 (02) : 1181 - 1194
12345