Rapid Concentration of Some Bacteriocin-like Compounds Using an Organic Solvent

被引：8

作者：

Chung, Dong-Min ^{[2
]}

Kim, Ki Eun ^{[1
,6
]}

Jeong, Seong-Yeop ^{[2
,5
]}

Park, Chang-Su ^{[3
]}

Ahn, Keug-Hyun ^{[2
]}

Kim, Dong Ho ^{[4
]}

Kang, Dae-Ook ^{[5
]}

Chun, Hyo Kon ^{[2
]}

Yoon, Byung-Dae ^{[2
]}

Koh, Hong Bum ^{[6
]}

Kim, Hong Jib ^{[1
]}

Choi, Nack-Shick ^{[1
]}

机构：

[1] Woori Life Sci Co Ltd, Cheonan 330858, Chungnam, South Korea

[2] KRIBB, Inst Bioind, Res Ctr, Jeongeup 580185, Jeonbuk, South Korea

[3] Catholic Univ Daegu, Dept Food Sci & Technol, Gyongsan 712702, Gyeongbuk, South Korea

[4] KAERI, Jeonbuk Branch Inst, Adv Radiat Technol Res Inst, Jeongeup 580185, Jeonbuk, South Korea

[5] Changwon Natl Univ, Dept Biochem & Hlth Sci, Chang Won 641773, Gyeongnam, South Korea

[6] Chonnam Natl Univ, Coll Vet Med, Kwangju 500757, South Korea

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2011年 / 20卷 / 05期

关键词：

bacteriocin-like compound; organic solvent; anti-microbial; Bacillus cereus; LACTIC-ACID BACTERIA; PURIFICATION; SEPARATION; GEL;

D O I：

10.1007/s10068-011-0201-8

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A new solvent extraction method for isolation of bacteriocin-like compounds (BLCs) from bacterial culture broth was developed. Culture supernatant of Pediococcus acidilactici WRL-1 was mixed with 3 volumes of cold acetone and maintained at -20 degrees C for 2 h. The solvent and precipitate were separated by centrifugation. Most (95.2 +/- 2.5%) pediocin activity was found in the liquid phase and it was obtained by evaporation. This method was also an effective means of isolating other BLCs from cultures of Lactobacillus acidophilus, Lactobacillus plantarum, and Bacillus licheniformis DJ-2. Other solvents (methanol, ethanol, and butanol) were also suitable toward this end.

引用

页码：1457 / 1459

页数：3

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