Application of semipreparative RP-18 HPLC for the purification of sesamin and sesamolin

被引：19
作者：
Amarowicz, R
Shahidi, F
Pegg, RB
机构：
[1] Polish Acad Sci, Inst Anim Reprod & Food Res, Div Food Sci, PL-10718 Olsztyn 5, Poland
[2] Mem Univ Newfoundland, Dept Biochem, St Johns, NF A1B 3X9, Canada
[3] Univ Saskatchewan, Dept Appl Microbiol & Food Sci, Saskatchewan Food Prod Innovat Program, Saskatoon, SK S7N 5A8, Canada
来源：
JOURNAL OF FOOD LIPIDS | 2001年 / 8卷 / 02期
关键词：
D O I：
10.1111/j.1745-4522.2001.tb00186.x
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A relatively simple chromatographic protocol was established to provide good separation of the unsaponifiable constituents from the seed oil of Sesamum indicum Linn. The protocol employs classical column chromatography using alumina and petroleum ether to collect furofuran lignans from sesame oil. Semipreparative RP-18 HPLC was used to separate the sesamin and sesamolin constituents in high purity (> 99%). The purity and identity of the separated compounds was confirmed by rechromatography using an analytical HPLC set up and GC-MS, respectively. The method described offers a means to isolate and collect sesamin and sesamolin in high purity for use as standards in furofuran lignan studies of sesame seed oil.
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页码：85 / 94
页数：10
相关论文
共 19 条
  • [1] RECENT RESEARCH ON SESAMIN SESAMOLIN + RELATED COMPOUNDS
    BUDOWSKI, P
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1964, 41 (04) : 280 - &
  • [2] CONTRIBUTION OF LIGNAN ANALOGS TO ANTIOXIDATIVE ACTIVITY OF REFINED UNROASTED SESAME SEED OIL
    FUKUDA, Y
    NAGATA, M
    OSAWA, T
    NAMIKI, M
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1986, 63 (08) : 1027 - 1031
  • [3] CHEMICAL ASPECTS OF THE ANTIOXIDATIVE ACTIVITY OF ROASTED SESAME SEED OIL, AND THE EFFECT OF USING THE OIL FOR FRYING
    FUKUDA, Y
    NAGATA, M
    OSAWA, T
    NAMIKI, M
    [J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (04): : 857 - 862
  • [4] FUKUDA Y, 1981, NIPPON SHOKUHIN KOGY, V28, P461
  • [5] THE CONSTITUENTS OF NATURAL PHENOLIC RESINS .23. THE CONSTITUTION OF SESAMOLIN
    HASLAM, E
    HAWORTH, RD
    [J]. JOURNAL OF THE CHEMICAL SOCIETY, 1955, : 827 - 833
  • [6] IKAN R, 1991, NATURAL PRODUCTS LAB, P47
  • [7] THIN-LAYER CHROMATOGRAPHIC SEPARATIONS OF SEED OIL UNSAPONIFIABLES FROM 4 SESAMUM SPECIES
    KAMALELDIN, A
    YOUSIF, G
    APPELQVIST, LA
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1991, 68 (11) : 844 - 847
  • [8] LIGNAN ANALYSIS IN SEED OILS FROM 4 SESAMUM SPECIES - COMPARISON OF DIFFERENT CHROMATOGRAPHIC METHODS
    KAMALELDIN, A
    APPELQVIST, LA
    YOUSIF, G
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1994, 71 (02) : 141 - 147
  • [9] SESAMINOL GLUCOSIDES IN SESAME SEEDS
    KATSUZAKI, H
    KAWAKISHI, S
    OSAWA, T
    [J]. PHYTOCHEMISTRY, 1994, 35 (03) : 773 - 776
  • [10] The use of sesame oil unsaponifiable matter as a natural antioxidant
    Mohamed, HMA
    Awatif, II
    [J]. FOOD CHEMISTRY, 1998, 62 (03) : 269 - 276
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