Inhibition of p42 and p44 mitogen-activated protein kinase activity by PD48059 does not suppress nerve growth factor-induced survival of sympathetic neurones

被引：0
|
作者：
Virdee, K [1 ]
Tolkovsky, AM [1 ]
机构：
[1] UNIV CAMBRIDGE,DEPT BIOCHEM,CAMBRIDGE CB2 1QW,ENGLAND
来源：
JOURNAL OF NEUROCHEMISTRY | 1996年 / 67卷 / 05期
基金：
英国惠康基金;
关键词：
cell death; apoptosis; p21; Ras; ciliary neurotrophic factor; leukemia inhibitory factor; cyclic AMP;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nerve growth factor (NGF) induces persistent p42 and p44 mitogen-activated protein kinase (MAPK) activity in sympathetic neurones in parallel to its survival-promoting activity. To investigate whether these MAPK activities are necessary for NGF-induced survival, we have inhibited NGF-stimulated p42/p44 MAPK activity over extended periods using the compound 2-(2'-amino-3'-methoxyphenyl)-oxanaphthalen-4-one (PD98059). Despite attaining up to 95% inhibition of p42/p44 MAPK activity in cultures treated with NGF and PD98059, neuronal survival is maintained undiminished, although a decrease in the density of the neuritic network is observed. Because p21Ras activity is essential for NGF-induced survival, we conclude that p21Ras-linked activities other than p42 and p44 MAPKs are responsible for mediating NGF-dependent survival of rat sympathetic neurones.
引用
收藏
页码：1801 / 1805
页数：5
相关论文
共 50 条
  • [31] Phosphorylation and activation of p42 and p44 mitogen-activated protein kinase are required for the P2 purinoceptor stimulation of endothelial prostacyclin production
    Patel, V
    Brown, C
    Goodwin, A
    Wilkie, N
    Boarder, MR
    BIOCHEMICAL JOURNAL, 1996, 320 : 221 - 226
  • [32] Regulation of iNOS by the p44/42 mitogen-activated protein kinase pathway in human melanoma
    J A Ellerhorst
    S Ekmekcioglu
    M K Johnson
    C P Cooke
    M M Johnson
    E A Grimm
    Oncogene, 2006, 25 : 3956 - 3962
  • [33] Regulation of iNOS by the p44/42 mitogen-activated protein kinase pathway in human melanoma
    Ellerhorst, J. A.
    Ekmekcioglu, S.
    Johnson, M. K.
    Cooke, C. P.
    Johnson, M. M.
    Grimm, E. A.
    ONCOGENE, 2006, 25 (28) : 3956 - 3962
  • [34] Diurnal variation in p42/44 mitogen-activated protein kinase in the rat pineal gland
    Ho, AK
    Mackova, M
    Price, L
    Chik, CL
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2003, 208 (1-2) : 23 - 30
  • [35] Lipopolysaccharide induces cholangiocyte proliferation via an interleukin-6-mediated activation of p44/p42 mitogen-activated protein kinase
    Park, J
    Gores, GJ
    Patel, T
    HEPATOLOGY, 1999, 29 (04) : 1037 - 1043
  • [36] P42/p44 mitogen-activated protein kinase activation is involved in prostaglandin F2α-induced interleukin-6 synthesis in osteoblasts
    Tokuda, H
    Kozawa, O
    Harada, A
    Uematsu, T
    CELLULAR SIGNALLING, 1999, 11 (05) : 325 - 330
  • [37] Vascular endothelial growth factor stimulates prostacyclin production and activation of cytosolic phospholipase A2 in endothelial cells via p42/p44 mitogen-activated protein kinase
    Wheeler-Jones, C
    Abu-Ghazaleh, R
    Cospedal, R
    Houliston, RA
    Martin, J
    Zachary, I
    FEBS LETTERS, 1997, 420 (01) : 28 - 32
  • [38] High glucose enhances cyclic strain-induced mesangial cell p44/p42 mitogen-activated protein kinase (MAPK) but not p38 MAPK
    Dlugosz, JA
    Whiteside, CI
    DIABETES, 1999, 48 : A137 - A137
  • [39] Delayed and sustained activation of p42/p44 mitogen-activated protein kinase induced by proteasome inhibitors through p21ras in PC12
    Hashimoto, K
    Guroff, G
    Katagiri, Y
    JOURNAL OF NEUROCHEMISTRY, 2000, 74 (01) : 92 - 98
  • [40] Involvement of p42/p44 mitogen-activated protein kinase in prostaglandin F2α-stimulated induction of heat shock protein 27 in osteoblasts
    Kozawa, O
    Tokuda, H
    Miwa, M
    Ito, H
    Matsuno, H
    Niwa, M
    Kato, K
    Uematsu, T
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1999, 75 (04) : 610 - 619
12345