The RING Finger Ubiquitin E3 Ligase SDIR1 Targets SDIR1-INTERACTING PROTEIN1 for Degradation to Modulate the Salt Stress Response and ABA Signaling in Arabidopsis

被引：144

作者：

Zhang, Huawei ^{[1
]}

Cui, Feng ^{[1
]}

Wu, Yaorong ^{[1
]}

Lou, Lijuan ^{[1
]}

Liu, Lijing ^{[1
]}

Tian, Miaomiao ^{[1
]}

Ning, Yuese ^{[1
]}

Shu, Kai ^{[1
]}

Tang, Sanyuan ^{[1
]}

Xie, Qi ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Gen, Beijing 100101, Peoples R China

来源：

PLANT CELL | 2015年 / 27卷 / 01期

基金：

美国国家科学基金会;

关键词：

FLUORESCENCE PROTEASE PROTECTION; KINASE-PHOSPHATASE PAIR; ABSCISIC-ACID; TRANSCRIPTION FACTOR; FUNCTIONAL-ANALYSIS; PLANT DEVELOPMENT; GENE-EXPRESSION; AUXIN RECEPTOR; FPP ASSAY; FAMILY;

D O I：

10.1105/tpc.114.134163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The plant hormone abscisic acid (ABA) regulates many aspects of plant development and the stress response. The intracellular E3 ligase SDIR1 (SALT- AND DROUGHT-INDUCED REALLY INTERESTING NEW GENE FINGER1) plays a key role in ABA signaling, regulating ABA-related seed germination and the stress response. In this study, we found that SDIR1 is localized on the endoplasmic reticulum membrane in Arabidopsis thaliana. Using cell biology, molecular biology, and biochemistry approaches, we demonstrated that SDIR1 interacts with and ubiquitinates its substrate, SDIRIP1 (SDIR1-INTERACTING PROTEIN1), to modulate SDIRIP1 stability through the 26S proteasome pathway. SDIRIP1 acts genetically downstream of SDIR1 in ABA and salt stress signaling. In detail, SDIRIP1 selectively regulates the expression of the downstream basic region/leucine zipper motif transcription factor gene ABA-INSENSITIVE5, rather than ABA-RESPONSIVE ELEMENTS BINDING FACTOR3 (ABF3) or ABF4, to regulate ABA-mediated seed germination and the plant salt response. Overall, the SDIR1/SDIRIP1 complex plays a vital role in ABA signaling through the ubiquitination pathway.

引用

页码：214 / 227

页数：14

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