PCR-SSCP:: A simple method for the authentication of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets

被引：23

作者：

Asensio, L ^{[1
]}

González, I ^{[1
]}

Fernández, A ^{[1
]}

Rodríguez, MA ^{[1
]}

Hernández, PE ^{[1
]}

García, T ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr & Bramatol Higiene & Tecnol Alimentos 3, E-28040 Madrid, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2001年 / 49卷 / 04期

关键词：

fish species identification; PCR-SSCP; 12S rRNA gene; Epinephelus guaza; Polyprion americanus; Lates niloticus;

D O I：

10.1021/jf001185w

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A method of DNA analysis has been developed to verify the authenticity of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. A short fragment (208 bp) of the mitochondrial 12S rRNA gene was amplified by the polymerase chain reaction and analyzed by single-strand conformation polymorphism to get species-specific patterns of single-stranded DNA (ssDNA). DNA strands were separated by native polyacrylamide gel electrophoresis and visualized by silver staining. Discrimination among the three fish species studied was possible, because each one expressed a specific ssDNA pattern.

引用

页码：1720 / 1723

页数：4

共 35 条

[1] ELECTROPHORETIC IDENTIFICATION OF FISH SPECIES USED IN SURIMI PRODUCTS [J].