A cell-free ROS-responsive hydrogel/oriented poly(lactide-co-glycolide) hybrid scaffold for reducing inflammation and restoring full-thickness cartilage defects in vivo

被引：17

作者：

Wu, Xinyu ^{[1
]}

Ding, Jie ^{[1
]}

Xu, Peifang ^{[2
]}

Feng, Xue ^{[1
]}

Wang, Zhaoyi ^{[1
]}

Zhou, Tong ^{[1
]}

Tu, Chenxi ^{[1
]}

Cao, Wangbei ^{[1
]}

Xie, Jieqi ^{[1
]}

Deng, Liwen ^{[1
]}

Shen, Liyin ^{[1
]}

Zhu, Yang ^{[1
]}

Gou, Zhongru ^{[3
]}

Gao, Changyou ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Polymer Sci & Engn, MOE Key Lab Macromol Synth & Functionalizat, Hangzhou 310027, Peoples R China

[2] Zhejiang Univ, Sch Med, Dept Ophthalmol, Affiliated Hosp 2, Hangzhou 310009, Peoples R China

[3] Zhejiang Univ, Zhejiang California Int Nanosyst Inst, Bionanomat & Regenerat Med Res Div, Hangzhou 310058, Peoples R China

来源：

BIOMEDICAL MATERIALS | 2021年 / 16卷 / 06期

关键词：

hydrogel; scaffold; ROS-responsive; cartilage regeneration; poly(lactide-co-glycolide); HYALURONIC-ACID; HYDROGEL; REGENERATION; REPAIR; GELATIN; MATRIX; NANOPARTICLES; EFFICIENT; CONSTRUCT; POLYMER;

D O I：

10.1088/1748-605X/ac21dd

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The modulation of inflammation in tissue microenvironment takes an important role in cartilage repair and regeneration. In this study, a novel hybrid scaffold was designed and fabricated by filling a reactive oxygen species (ROS)-scavenging hydrogel (RS Gel) into a radially oriented poly(lactide-co-glycolide) (PLGA) scaffold. The radially oriented PLGA scaffolds were fabricated through a temperature gradient-guided phase separation and freeze-drying method. The RS Gel was formed by crosslinking the mixture of ROS-responsive hyperbranched polymers and biocompatible methacrylated hyaluronic acid (HA-MA). The hybrid scaffolds exhibited a proper compressive modulus, good ROS-scavenging capability, and cell compatibility. In vivo tests showed that the hybrid scaffolds significantly regulated inflammation and promoted regeneration of hyaline cartilage after they were implanted into full-thickness cartilage defects in rabbits for 12 w. In comparison with the PLGA scaffolds, the neo-cartilage in the hybrid scaffolds group possessed more deposition of glycosaminoglycans and collagen type II, and were well integrated with the surrounding tissue.

引用

页数：15

共 2 条

[1] Regeneration of osteochondral defects in vivo by a cell-free cylindrical poly(lactide-co-glycolide) scaffold with a radially oriented microstructure
Dai, Yuankun
Shen, Tao
Ma, Lie
Wang, Dongan
Gao, Changyou
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2018, 12 (03) : E1647 - E1661
[2] In vivo restoration of full-thickness cartilage defects by poly(lactide-co-glycolide) sponges filled with fibrin gel, bone marrow mesenchymal stem cells and DNA complexes
Wang, Wei
Li, Bo
Li, Yanglin
Jiang, Yangzi
Ouyang, Hongwei
Gao, Changyou
BIOMATERIALS, 2010, 31 (23) : 5953 - 5965

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