Mechanism of positioning the cell nucleus in vertebrate photoreceptors

被引:72
作者
Tsujikawa, Motokazu [1 ]
Omori, Yoshihiro [1 ]
Biyanwila, Janisha [1 ]
Malicki, Jarema [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Ophthalmol, Boston, MA 02114 USA
关键词
Bardet-Biedl syndrome; retina; motor complex; neuroclegeneration;
D O I
10.1073/pnas.0700178104
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Organelles are frequently distributed in a nonrandom manner in a cell's cytoplasm. A particular distribution pattern often facilitates a specific function of a cell, whereas its aberrations can lead to cell death. We show that a mutation in the zebrafish mikre oko (mok) locus, which encodes dynactin 1 subunit of the dynactin complex, produces a severe displacement of the photoreceptor cell nucleus toward the synaptic terminus. Interference with the function of other dynein complex constituents, including p50/dynamitin, the Lis1 polypeptide, and the disruption of a nuclear envelope component of the syne gene family in vertebrate photoreceptors also result in the mispositioning of nuclei. Although the overall photoreceptor polarity is not affected, this phenotype is accompanied by a misdistribution of the Bardet-Biedl syndrome 4 polypeptide and a decreased photoreceptor survival. These findings reveal an important mechanism that regulates nuclear position in vertebrate neurons.
引用
收藏
页码:14819 / 14824
页数:6
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