In vitro and in vivo anti-tumor efficiency comparison of phosphorylcholine micelles with PEG micelles

被引:34
作者
Cai, Mengtan [1 ]
Cao, Jun [2 ]
Wu, Zhengzhong [1 ]
Cheng, Furong [1 ]
Chen, Yuanwei [1 ]
Luo, Xianglin [1 ,3 ]
机构
[1] Sichuan Univ, Coll Polymer Sci & Engn, Chengdu 610065, Sichuan, Peoples R China
[2] Sichuan Univ, Natl Engn Res Ctr Biomat, Chengdu, Sichuan, Peoples R China
[3] Sichuan Univ, State Key Lab Polymer Mat Engn, Chengdu, Sichuan, Peoples R China
基金
中国国家自然科学基金;
关键词
Zwitterion; Phosphorylcholine; Poly(ethylene glycol); Antitumor efficiency; Nanoparticle; Drug delivery; SELF-ASSEMBLED MONOLAYERS; BLOCK-COPOLYMER MICELLES; CELLULAR UPTAKE; DRUG-DELIVERY; POLY(2-METHACRYLOYLOXYETHYL PHOSPHORYLCHOLINE); MULTIDRUG-RESISTANCE; CANCER; WATER; FUNCTIONALIZATION; NANOPARTICLES;
D O I
10.1016/j.colsurfb.2017.05.053
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Polymer micelles for anticancer drug delivery have shown many advantages. In this study, poly(epsilon-caprolactone)-b-poly(2-methacryloyloxyethyl phosphorylcholine) (PCL-PMPC) with bio-inspired structure self-assembled into small and uniform micelles as traditional poly(epsilon-caprolactone)-b-poly(ethylene glycol) (PCL-PEG). The in vitro and in vivo anti-tumor efficiency of PCL-PMPC and PCL-PEG micelles were detailedly evaluated. The both micelles were able to load DOX with high efficiency. PCL-PMPC micelles exhibited faster drug release at pH 5.5 than that of PCL-PEG micelles. Confocal laser scanning microscopy and flow cytometry results showed that PCL-PMPC micelles were more effectively internalized by tumor cells. DOX-loaded PCL-PMPC micelles presented higher cytotoxicity to tumor cells. PCL-PMPC micelles displayed not only longer circulation time in pharmacokinetics investigation, but also higher accumulation at the tumor site in in vivo imaging study in comparison with PCL-PEG micelles. More importantly, in a tumor model DOX-loaded PCL-PMPC micelles showed better therapeutic efficacy than DOX-loaded PCL-PEG micelles along with mild side effects. Therefore, PCL-PMPC micelles are deemed to be promising drug carriers for cancer therapy. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:268 / 279
页数:12
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