Extracellular glucose level regulates dependence on GRP78 for cell surface localization of multipass transmembrane proteins in HeLa cells

被引:7
作者
Toyoda, Yusuke [1 ]
Akarlar, Busra [2 ]
Sarov, Mihail [3 ]
Ozlu, Nurhan [2 ]
Saitoh, Shigeaki [1 ]
机构
[1] Kurume Univ, Inst Life Sci, Asahi Machi 67, Kurume, Fukuoka 8300011, Japan
[2] Koc Univ, Dept Mol Biol & Genet, Istanbul, Turkey
[3] Max Planck Inst Mol Cell Biol & Genet, Dresden, Germany
基金
日本学术振兴会;
关键词
chaperone; endoplasmic reticulum; glucose starvation; GLUT1; GRP78; ENDOPLASMIC-RETICULUM; MOLECULAR CHAPERONES; PATHWAY; TRANSPORT; STRESS; FAMILY; EXPRESSION; CALRETICULIN; STIMULATION; MECHANISMS;
D O I
10.1002/1873-3468.13232
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many human-cultured cell lines survive glucose starvation, but the underlying mechanisms remain unclear. Here, we searched for proteins required for cellular adaptation to glucose-limited conditions and identified several endoplasmic reticulum chaperones in the glucose-regulated protein (GRP) family as proteins enriched in the cellular membrane. Surprisingly, these proteins, which are required for cell surface localization of GLUT1 under high-glucose conditions, become dispensable for targeting GLUT1 to the surface upon glucose starvation. In marked contrast, cell surface localization of single-pass transmembrane proteins, such as epidermal growth factor receptor and CD98, is not disturbed by GRP78 depletion regardless of the extracellular glucose level. These results indicate that the extracellular glucose level regulates dependence on the GRPs for cell surface localization of multipass transmembrane proteins.
引用
收藏
页码:3295 / 3304
页数:10
相关论文
共 48 条
  • [1] Maximum yields of microsomal-type membranes from small amounts of plant material without requiring ultracentrifugation
    Abas, Lindy
    Luschnig, Christian
    [J]. ANALYTICAL BIOCHEMISTRY, 2010, 401 (02) : 217 - 227
  • [2] The Protein Family of Glucose Transport Facilitators: It's Not Only About Glucose After All
    Augustin, Robert
    [J]. IUBMB LIFE, 2010, 62 (05) : 315 - 333
  • [3] UniProt: the universal protein knowledgebase
    Bateman, Alex
    Martin, Maria Jesus
    O'Donovan, Claire
    Magrane, Michele
    Alpi, Emanuele
    Antunes, Ricardo
    Bely, Benoit
    Bingley, Mark
    Bonilla, Carlos
    Britto, Ramona
    Bursteinas, Borisas
    Bye-A-Jee, Hema
    Cowley, Andrew
    Da Silva, Alan
    De Giorgi, Maurizio
    Dogan, Tunca
    Fazzini, Francesco
    Castro, Leyla Garcia
    Figueira, Luis
    Garmiri, Penelope
    Georghiou, George
    Gonzalez, Daniel
    Hatton-Ellis, Emma
    Li, Weizhong
    Liu, Wudong
    Lopez, Rodrigo
    Luo, Jie
    Lussi, Yvonne
    MacDougall, Alistair
    Nightingale, Andrew
    Palka, Barbara
    Pichler, Klemens
    Poggioli, Diego
    Pundir, Sangya
    Pureza, Luis
    Qi, Guoying
    Rosanoff, Steven
    Saidi, Rabie
    Sawford, Tony
    Shypitsyna, Aleksandra
    Speretta, Elena
    Turner, Edward
    Tyagi, Nidhi
    Volynkin, Vladimir
    Wardell, Tony
    Warner, Kate
    Watkins, Xavier
    Zaru, Rossana
    Zellner, Hermann
    Xenarios, Ioannis
    [J]. NUCLEIC ACIDS RESEARCH, 2017, 45 (D1) : D158 - D169
  • [4] STIMULATION OF SYSTEM Y+-LIKE AMINO-ACID-TRANSPORT BY THE HEAVY-CHAIN OF HUMAN 4F2 SURFACE-ANTIGEN IN XENOPUS-LAEVIS OOCYTES
    BERTRAN, J
    MAGAGNIN, S
    WERNER, A
    MARKOVICH, D
    BIBER, J
    TESTAR, X
    ZORZANO, A
    KUHN, LC
    PALACIN, M
    MURER, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) : 5606 - 5610
  • [5] Endoplasmic Reticulum and the Unfolded Protein Response: Dynamics and Metabolic Integration
    Bravo, Roberto
    Parra, Valentina
    Gatica, Damian
    Rodriguez, Andrea E.
    Torrealba, Natalia
    Paredes, Felipe
    Wang, Zhao V.
    Zorzano, Antonio
    Hill, Joseph A.
    Jaimovich, Enrique
    Quest, Andrew F. G.
    Lavandero, Sergio
    [J]. INTERNATIONAL REVIEW OF CELL AND MOLECULAR BIOLOGY, VOL 301, 2013, 301 : 215 - 290
  • [6] Regulated transport of the glucose transporter glut4
    Bryant, NJ
    Govers, R
    James, DE
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2002, 3 (04) : 267 - 277
  • [7] Cai B, 1998, J CELL PHYSIOL, V177, P282, DOI 10.1002/(SICI)1097-4652(199811)177:2<282::AID-JCP10>3.0.CO
  • [8] 2-C
  • [9] CARPENTER G, 1978, NATURE, V276, P409, DOI 10.1038/276409a0
  • [10] Glucose-Regulated Protein 78 (GRP78) Silencing Enhances Cell Migration but Does Not Influence Cell Proliferation in Hepatocellular Carcinoma
    Chang, Yu-Jia
    Chiu, Chong-Chi
    Wu, Chih-Hsiung
    An, Jane
    Wu, Cheng-Chia
    Liu, Tsan-Zon
    Wei, Po-Li
    Huang, Ming-Te
    [J]. ANNALS OF SURGICAL ONCOLOGY, 2010, 17 (06) : 1703 - 1709