Impact of phenolic compounds in the acyl homoserine lactone-mediated quorum sensing regulatory pathways

被引：68

作者：

Hossain, Md. Akil ^{[1
,2
]}

Lee, Seung-Jin ^{[1
]}

Park, Na-Hye ^{[1
]}

Mechesso, Abraham Fikru ^{[1
]}

Birhanu, Biruk Tesfaye ^{[1
]}

Kang, JeongWoo ^{[2
]}

Reza, Md. Ahsanur ^{[3
]}

Suh, Joo-Won ^{[4
]}

Park, Seung-Chun ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Coll Vet Med, Lab Clin Pharmacokinet & Pharmacodynam, Daegu 702701, South Korea

[2] Anim & Plant Quarantine Agcy QIA, Vet Drugs & Biol Div, 177,Hyeoksin 8-Ro, Gimcheon Si 39660, Gyeongsangbuk D, South Korea

[3] Patuakhali Sci & Technol Univ, Fac Anim Sci & Vet Med, Outer Campus, Babugonj 8210, Barisal, Bangladesh

[4] Myongji Univ, Div Biosci & Bioinformat, Ctr Nutraceut & Pharmaceut Mat, Sci Campus, Yongin 449728, Gyeonggi, South Korea

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

新加坡国家研究基金会;

关键词：

PSEUDOMONAS-AERUGINOSA VIRULENCE; SMALL-MOLECULE INHIBITORS; SWARMING MOTILITY; CHROMOBACTERIUM-VIOLACEUM; ANTIBIOTIC-RESISTANCE; INDUCED CYTOTOXICITY; METHYL GALLATE; GROWTH-PHASE; IN-VITRO; BIOFILM;

D O I：

10.1038/s41598-017-10997-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Quorum sensing (QS) is a cell density-dependent regulation of virulent bacterial gene expression by autoinducers that potentially pertains in the epidemic of bacterial virulence. This study was initially designed to evaluate the effect of 5 phenolic compounds in the modulation of QS and virulence factors of Chromobacterium violaceum and Pseudomonas aeruginosa, and to determine the mechanisms of their effects. Biosensor strains were used to assess antibacterial and anti-QS effect of these compounds. Only methyl gallate (MG) among these compounds demonstrated profound anti-QS effect in the preliminary study, and thus only MG was utilized further to evaluate the effects on the synthesis and activity of acyl homoserine lactone (AHL) in C. violaceum and on the modulation of biofilm, motility, proteolytic, elastase, pyocyanin, and rhamnolipid activity in P. aeruginosa. Finally, the effect of MG on the expression of QS-regulated genes of P. aeruginosa was verified. MG suppressed both the synthesis and activity of AHL in C. violaceum. It also restricted the biofilm formation and other QS-associated virulence factor of P. aeruginosa. MG concentration-dependently suppressed the expression of lasI/R, rhlI/R, and pqsA of P. aeruginosa and was non-toxic in in vitro study. This is the first report of the anti-QS mechanism of MG.

引用

页数：16

共 97 条

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