Evaluation of APOBEC3B Recognition Motifs by NMR Reveals Preferred Substrates

被引：10

作者：

Liu, Manjuan ^{[1
]}

Mallinger, Aurelie ^{[1
]}

Tortorici, Marcello ^{[1
]}

Newbatt, Yvette ^{[1
]}

Richards, Meirion ^{[1
]}

Mirza, Amin ^{[1
]}

van Montfort, Rob L. M. ^{[1
]}

Burke, Rosemary ^{[1
]}

Blagg, Julian ^{[1
]}

Kaserer, Teresa ^{[1
]}

机构：

[1] Inst Canc Res, Canc Res UK Canc Therapeut Unit, London SM2 5NG, England

来源：

ACS CHEMICAL BIOLOGY | 2018年 / 13卷 / 09期

关键词：

DNA CYTOSINE; CATALYTIC DOMAIN; STRUCTURAL BASIS; HUMAN CANCERS; DEAMINATION; MUTAGENESIS; RESTRICTION; MUTATION; BINDING;

D O I：

10.1021/acschembio.8b00639

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

APOBEC3B (A3B) deamination activity on ssDNA is considered a contributing factor to tumor heterogeneity and drug resistance in a number of human cancers. Despite its clinical impact, little is known about A3B ssDNA substrate preference. We have used nuclear magnetic resonance to monitor the catalytic turnover of A3B substrates in real-time. This study reports preferred nucleotide sequences for A3B substrates, including optimized 4-mer oligonucleotides, and reveals a breadth of substrate recognition that includes DNA sequences known to be mutated in drug-resistant cancer clones. Our results are consistent with available clinical and structural data and may inform the design of substrate-based A3B inhibitors.

引用

页码：2427 / 2432

页数：6

共 50 条

[31] APOBEC3B: Pathological consequences of an innate immune DNA mutator [J].

Burns, Michael B. ;

Leonard, Brandon ;

Harris, Reuben S. .

BIOMEDICAL JOURNAL, 2015, 38 (02) :102-110

[32] Classical NF-kB pathway is responsible for APOBEC3B expression in cancer cells [J].

Maruyama, Wataru ;

Shirakawa, Kotaro ;

Matsui, Hiroyuki ;

Matsumoto, Tadahiko ;

Yamazaki, Hiroyuki ;

Sarca, Anamaria D. ;

Kazuma, Yasuhiro ;

Kobayashi, Masayuki ;

Shindo, Keisuke ;

Takaori-Kondo, Akifumi .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2016, 478 (03) :1466-1471

[33] APOBEC3B lysine residues are dispensable for DNA cytosine deamination, HIV-1 restriction, and nuclear localization [J].

Molan, Amy M. ;

Hanson, Heather M. ;

Chweya, Cynthia M. ;

Anderson, Brett D. ;

Starrett, Gabriel J. ;

Richards, Christopher M. ;

Harris, Reuben S. .

VIROLOGY, 2017, 511 :74-81

[34] Identification of APOBEC3B promoter elements responsible for activation by human papillomavirus type 16 E6 [J].

Mori, Seiichiro ;

Takeuchi, Takamasa ;

Ishii, Yoshiyuki ;

Kukimoto, Iwao .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2015, 460 (03) :555-560

[35] The Impact of Sugar Conformation on the Single-Stranded DNA Selectivity of APOBEC3A and APOBEC3B Enzymes [J].

Wyllie, Mackenzie K. ;

Morris, Clare K. ;

Moeller, Nicholas H. ;

Schares, Henry A. M. ;

Moorthy, Ramkumar ;

Belica, Christopher A. ;

Grillo, Michael J. ;

Demir, Ozlem ;

Ayoub, Alex M. ;

Carpenter, Michael A. ;

Aihara, Hideki ;

Harris, Reuben S. ;

Amaro, Rommie E. ;

Harki, Daniel A. .

ACS CHEMICAL BIOLOGY, 2024, 20 (01) :117-127

[36] Expression of APOBEC3B mRNA in Primary Breast Cancer of Japanese Women [J].

Tokunaga, Eriko ;

Yamashita, Nami ;

Tanaka, Kimihiro ;

Inoue, Yuka ;

Akiyoshi, Sayuri ;

Saeki, Hiroshi ;

Oki, Eiji ;

Kitao, Hiroyuki ;

Maehara, Yoshihiko .

PLOS ONE, 2016, 11 (12)

[37] DNA replication stress: a source of APOBEC3B expression in breast cancer [J].

Cescon, David W. ;

Haibe-Kains, Benjamin .

GENOME BIOLOGY, 2016, 17

[38] Protein kinase A inhibits tumor mutator APOBEC3B through phosphorylation [J].

Matsumoto, Tadahiko ;

Shirakawa, Kotaro ;

Yokoyama, Masaru ;

Fukuda, Hirofumi ;

Sarca, Anamaria Daniela ;

Koyabu, Sukenao ;

Yamazaki, Hiroyuki ;

Kazuma, Yasuhiro ;

Matsui, Hiroyuki ;

Maruyama, Wataru ;

Nagata, Kayoko ;

Tanabe, Fumiko ;

Kobayashi, Masayuki ;

Shindo, Keisuke ;

Morishita, Ryo ;

Sato, Hironori ;

Takaori-Kondo, Akifumi .

SCIENTIFIC REPORTS, 2019, 9 (1)

[39] Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity [J].

Jin, Shuai ;

Fei, Hongyuan ;

Zhu, Zixu ;

Luo, Yingfeng ;

Liu, Jinxing ;

Gao, Shenghan ;

Zhang, Feng ;

Chen, Yu-Hang ;

Wang, Yanpeng ;

Gao, Caixia .

MOLECULAR CELL, 2020, 79 (05) :728-+

[40] APOBEC3B up-regulation independently predicts ovarian cancer prognosis: a cohort study [J].

Du, Yan ;

Tao, Xiang ;

Wu, Jing ;

Yu, Huandi ;

Yu, Yinhua ;

Zhao, Hongbo .

CANCER CELL INTERNATIONAL, 2018, 18

← 1 2 3 4 5 →