Antibiotic resistance and population structure of cystic fibrosis Pseudomonas aeruginosa isolates from a Spanish multi-centre study

被引：24

作者：

Lopez-Causape, Carla ^{[1
,2
,3
]}

de Dios-Caballero, Juan ^{[3
,4
,5
]}

Cobo, Marta ^{[4
,5
]}

Escribano, Amparo ^{[6
,7
]}

Asensio, Oscar ^{[8
]}

Oliver, Antonio ^{[1
,2
,3
]}

del Campo, Rosa ^{[3
,4
,5
]}

Canton, Rafael ^{[3
,4
,5
]}

机构：

[1] Hosp Univ Son Espases, Inst Invest Sanitaria Illes Balears, Serv Microbiol, Palma De Mallorca, Spain

[2] Hosp Univ Son Espases, Inst Invest Sanitaria Illes Balears, Unidad Invest, Palma De Mallorca, Spain

[3] Inst Salud Carlos III, Red Espanola Invest Patol Infecciosa, Madrid, Spain

[4] Hosp Univ Ramon & Cajal, Serv Microbiol, Ctra Colmenar,Km 9,1, Madrid 28034, Spain

[5] Inst Ramon & Cajal Invest Sanitaria, Madrid, Spain

[6] Hosp Clin Univ, Serv Pediat, Unidad Neumol Pediat & Fibrosis Quist, Valencia, Spain

[7] Univ Valencia, Valencia, Spain

[8] Hosp Univ Sabadell, Unidad Neumol & Alergia Pediat, Corp Sanit Parc Tauli, Barcelona, Spain

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2017年 / 50卷 / 03期

关键词：

Antibiotic resistance; Virulence; Multi-locus sequence typing (MLST); Array tube; Cystic fibrosis; Pseucforrumus aeruginosa; LUNG INFECTIONS; ANTIMICROBIAL SUSCEPTIBILITY; STRAINS; ADAPTATION; HOSPITALS; DIVERSITY; DISEASE; GENES;

D O I：

10.1016/j.ijantimicag.2017.03.034

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The first Spanish multi-centre study on the microbiology of cystic fibrosis (CF) was conducted from 2013 to 2014. The study involved 24 CF units from 17 hospitals, and recruited 341 patients. The aim of this study was to characterise Pseudomonas aeruginosa isolates, 79 of which were recovered from 75 (22%) patients. The study determined the population structure, antibiotic susceptibility profile and genetic background of the strains. Fifty-five percent of the isolates were multi-drug-resistant, and 16% were extensively drug-resistant. Defective mutS and mutL genes were observed in mutator isolates (15.2%). Considerable genetic diversity was observed by pulsed-field gel electrophoresis (70 patterns) and multi-locus sequence typing (72 sequence types). International epidemic clones were not detected. Fifty-one new and 14 previously described array tube (AT) genotypes were detected by AT technology. This study found a genetically unrelated and highly diverse CF P. aeruginosa population in Spain, not represented by the epidemic clones widely distributed across Europe, with multiple combinations of virulence factors and high antimicrobial resistance rates (except for colistin). (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

引用

页码：334 / 341

页数：8

共 37 条

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