Functional significance of octameric RuvA for a branch migration complex from Thermus thermophilus

被引:6
作者
Fujiwara, Yoshie [2 ]
Mayanagi, Kouta [1 ,3 ,4 ]
Morikawa, Kosuke [1 ,5 ]
机构
[1] Osaka Univ, Inst Prot Res, Takara Bio Endowed Div, Osaka 5650874, Japan
[2] Osaka Univ, Grad Sch Engn Sci, Osaka 5608531, Japan
[3] Nagahama Inst Biosci & Technol, Shiga 5260829, Japan
[4] JST BIRD, Nagahama 5260829, Japan
[5] JST, CREST, Chiyoda Ku, Tokyo 1020075, Japan
关键词
RuvAB; branch migration; Holliday junction; octameric RuvA structure;
D O I
10.1016/j.bbrc.2007.11.149
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The RuvAB complex promotes migration of Holliday junction at the late stage of homologous recombination. The RuvA tetramer specifically recognizes Holliday junction to form two types of complexes. A single tetramer is bound to the open configuration of the junction DNA in complex I, while the octameric RuvA core structure sandwiches the same junction in complex II. The hexameric RuvB rings, symmetrically bound to both sides of RuvA on Holliday junction, pump out DNA duplexes, depending upon ATP hydrolysis. We investigated functional differences between the wild-type RuvA from Thermus thermophilus and mutants impaired the ability of complex II formation. These mutant RuvA, exclusively forming complex I, reduced activities of branch migration and ATP hydrolysis, suggesting that the octameric RuvA is essential for efficient branch migration. Together with our recent electron microscopic analysis, this finding provides important insights into functional roles of complex II in the coordinated branch migration mechanism. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:426 / 431
页数:6
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