Development of sensitive and fast immunoassays for amantadine detection

被引：44
作者：
Xu, Liguang [1 ,2 ]
Peng, Shuang [1 ,2 ]
Liu, Liqiang [1 ,2 ]
Song, Shanshan [1 ,2 ]
Kuang, Hua [1 ,2 ]
Xu, Chuanlai [1 ,2 ]
机构：
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2016年 / 27卷 / 05期
关键词：
Amantadine; chicken samples; ic-ELISA; immunochromatographic strip; IMMUNOCHROMATOGRAPHIC ASSAY; PRECOLUMN DERIVATIZATION; STRIP TEST; ANTIBODY; PLASMA;
D O I：
10.1080/09540105.2016.1148667
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A sensitive indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) method and a gold nanoparticle immunochromatographic strip were developed to detect amantadine (AM) in foods. A novel AM hapten was prepared, and a sensitive monoclonal antibody against AM was developed. The optimum ic-ELISA conditions included a pH of 7.4 and an ionic strength of 1.6% with no organic solvents. The half-inhibition concentration (IC50) value of ic-ELISA was 1.92ng/ml, with a limit of detection of 0.62ng/ml. The immunochromatographic test strip method had a visual cut-off value at 5g/kg. Chicken samples were spiked with three concentrations of AM (1, 2, and 5 mu g/kg) and analysed by ic-ELISA. Good recoveries were obtained (92.3% at 1 mu g/kg AM, 116.1% at 2 mu g/kg AM, and 91.8% at 5 mu g/kg AM).
引用
收藏
页码：678 / 688
页数：11
相关论文
共 29 条
  • [1] Development of a rapid and sensitive enzyme-linked immunosorbent assay to detect clonidine residues in swine urine samples
    Bai, Yuhui
    Liu, Zhihong
    Wang, Hejia
    Bi, Yanfeng
    Huang, Yaoling
    Sun, Lei
    Su, Fuqin
    Jin, Yinzhen
    Zhang, Congmin
    Xu, Shixin
    [J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 2015, 26 (01) : 1 - 12
  • [2] Synthesis of a newly designed artificial antigen and preparation of a polyclonal antibody against salbutamol
    Chen, Guanyin
    Huang, Xianhui
    Li, Shuaipeng
    Kong, Xiangkai
    Huai, Binbin
    [J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 2014, 25 (03) : 322 - 331
  • [3] Antibody development to testosterone and its application in capillary electrophoresis-based immunoassay
    Chen, Hong-Xu
    Zhang, Xin-Xiang
    [J]. ELECTROPHORESIS, 2008, 29 (16) : 3406 - 3413
  • [4] General immunoassay for pyrethroids based on a monoclonal antibody
    Chen, Xiujin
    Xu, Liguang
    Ma, Wei
    Liu, Liqiang
    Kuang, Hua
    Wang, Libing
    Xu, Chuanlai
    [J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 2014, 25 (03) : 341 - 349
  • [5] Fluorimetric liquid chromatographic analysis of amantadine in urine and pharmaceutical formulation
    Duh, TH
    Wu, HL
    Pan, CW
    Kou, HS
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2005, 1088 (1-2) : 175 - 181
  • [6] Development of an ELISA for nitrazepam based on a monoclonal antibody
    Guan, Dandan
    Guo, Lingling
    Liu, Liqiang
    Kong, Na
    Kuang, Hua
    Xu, Chuanlai
    [J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 2015, 26 (05) : 611 - 621
  • [7] Development of a monoclonal antibody-based immunochromatographic strip for cephalexin
    Guo, Jingnan
    Liu, Liqiang
    Xue, Feng
    Xing, Changrui
    Song, Shanshan
    Kuang, Hua
    Xu, Chuanlai
    [J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 2015, 26 (02) : 282 - 292
  • [8] Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent
    Higashi, Y
    Nakamura, S
    Matsumura, H
    Fujii, Y
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2006, 20 (05) : 423 - 428
  • [9] Simultaneous determination of amantadine and rimantadine by HPLC in rat plasma with pre-column derivatization and fluorescence detection for pharmacokinetic studies
    Higashi, Y
    Uemori, I
    Fujii, Y
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2005, 19 (09) : 655 - 662
  • [10] Liquid chromatographic determination of 1-adamantanamine and 2-adamantanamine in human plasma after pre-column derivatization with o-phthalaldehyde and 1-thio-β-D-glucose
    Higashi, Y
    Fujii, Y
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 799 (02): : 349 - 354
123