Continuous fluorometric assays for acetylcholinesterase activity and inhibition with conjugated polyelectrolytes

被引：153
作者：
Feng, Fude [1 ]
Tang, Yanli [1 ]
Wang, Shu [1 ]
Li, Yuliang [1 ]
Zhu, Daoben [1 ]
机构：
[1] Chinese Acad Sci, Inst Chem, Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100080, Peoples R China
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 41期
关键词：
biosensors; conjugated polymers; drug screening; enzyme catalysis; fluorescent probes;
D O I：
10.1002/anie.200701724
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Quick but sensitive: A super-quenched fluorogenic complex of a cationic acetylcholine (ACh) derivative with an energy-acceptor tag and an anionic water-soluble conjugated polymer forms the basis of a highly effective fluorescence turn-on assay for studying the enzyme kinetics and inhibition of acetylcholinesterase (AChE; see schematic representation of the assay). FRET = fluorescence resonant energy transfer. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
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收藏
页码：7882 / 7886
页数：5
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