Analysis of solid tumor mutation profiles in liquid biopsy

被引：19

作者：

Balaji, Sai A. ^{[1
,2
]}

Shanmugam, Ashwini ^{[1
]}

Chougule, Anuradha ^{[3
]}

Sridharan, Srikant ^{[1
]}

Prabhash, Kumar ^{[3
]}

Arya, Anuradha ^{[2
]}

Chaubey, Aditya ^{[2
]}

Hariharan, Arun ^{[1
,2
]}

Kolekar, Pandurang ^{[1
]}

Sen, Manimala ^{[1
]}

Ravichandran, Aarthi ^{[1
]}

Katragadda, Shanmukh ^{[1
]}

Sankaran, Satish ^{[1
]}

Bhargava, Saurabh ^{[4
]}

Kulkarni, Prashanth ^{[4
]}

Rao, Suchitra ^{[4
]}

Sunkavalli, Chinnababu ^{[5
]}

Banavali, Shripad ^{[3
]}

Joshi, Amit ^{[3
]}

Noronha, Vanita ^{[3
]}

Dutt, Amit ^{[6
]}

Bahadur, Urvashi ^{[1
]}

Hariharan, Ramesh ^{[1
]}

Veeramachaneni, Vamsi ^{[1
]}

Gupta, Vaijayanti ^{[1
,2
]}

机构：

[1] Strand Life Sci Pvt Ltd, Bangalore, Karnataka, India

[2] Mazumdar Shaw Med Fdn, Mazumdar Shaw Ctr Translat Res, Bangalore, Karnataka, India

[3] Tata Mem Hosp, Bombay, Maharashtra, India

[4] Mazumdar Shaw Med Ctr, Bangalore, Karnataka, India

[5] Apollo Canc Specialty Hosp, Hyderabad, Telangana, India

[6] Tata Mem Hosp, Adv Ctr Treatment Res & Educ Canc, Raigad, Maharashtra, India

来源：

CANCER MEDICINE | 2018年 / 7卷 / 11期

关键词：

concordance; ctDNA; monitoring; prognosis; survival outcome; CELL-FREE DNA; CANCER PATIENTS; LUNG-CANCER; PLASMA;

D O I：

10.1002/cam4.1791

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Liquid biopsy is increasingly gaining traction as an alternative to invasive solid tumor biopsies for prognosis, treatment decisions, and disease monitoring. Matched tumor-plasma samples were collected from 180 patients across different cancers with >90% of the samples below Stage IIIB. Tumors were profiled using next-generation sequencing (NGS) or quantitative PCR (qPCR), and the mutation status was queried in the matched plasma using digital platforms such as droplet digital PCR (ddCPR) or NGS for concordance. Tumor-plasma concordance of 82% and 32% was observed in advanced (Stage IIB and above) and early (Stage I to Stage IIA) stage samples, respectively. Interestingly, the overall survival outcomes correlated to presurgical/at-biopsy ctDNA levels. Baseline ctDNA stratified patients into three categories: (a) high ctDNA correlated with poor survival outcome, (b) undetectable ctDNA with good outcome, and (c) low ctDNA whose outcome was ambiguous. ctDNA could be a powerful tool for therapy decisions and patient management in a large number of cancers across a variety of stages.

引用

页码：5439 / 5447

页数：9

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