Synthesis and characterization of folic acid-chitosan nanoparticles loaded with thymoquinone to target ovarian cancer cells

被引：35

作者：

Ince, Iskender ^{[1
,2
]}

Yildirim, Yeliz ^{[1
,3
]}

Guler, Gunnur ^{[4
]}

Medine, Emin Ilker ^{[2
]}

Ballica, Gulsah ^{[3
]}

Kusdemir, Bekir Cem ^{[5
]}

Goker, Erdem ^{[6
]}

机构：

[1] Ege Univ, Ctr Drug R&D & Pharmacokinet Applicat ARGEFAR, Izmir, Turkey

[2] Ege Univ, Inst Nucl Sci, Dept Nucl Applicat, Izmir, Turkey

[3] Ege Univ, Fac Sci, Dept Chem, Izmir, Turkey

[4] Izmir Univ Econ, Fac Engn, Dept Biomed Engn, Izmir, Turkey

[5] Ege Univ, Grad Sch Nat & Appl Sci, Dept Biotechnol, TR-35100 Izmir, Turkey

[6] Ege Univ, Fac Med, Div Med Oncol, Izmir, Turkey

来源：

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY | 2020年 / 324卷 / 01期

关键词：

Thymoquinone (TQ); Chitosan nanoparticles; Folate-receptor-targeted; Ovarian cancer; INFRARED-SPECTROSCOPY; FTIR SPECTROSCOPY; FOLATE RECEPTORS; DRUG-DELIVERY; APOPTOSIS; INHIBITION; PROTEIN; AGENT;

D O I：

10.1007/s10967-020-07058-z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The aim of the present research was to formulate and characterize radioiodinated folic acid-chitosan conjugated thymoquinone nanoparticles (FATQCSNPs) and to increase targeting ability on ovarian cancer cell. The dose of drug-loading into the FATQCSNPs and the amount of folic acid on the FATQCSNPs surface were determined as a 20.0 +/- 1% and 46.0 +/- 0.5%, respectively. Cell viabilities (%) determined on SKOV-3 and Caco-2 cells for 48 h. TQ, TQCS and FATQCS were very cytotoxic with lower IC50 values on both cell lines. At specific-activity-dependent incorporation study, the incorporation efficiencies of I-131-FATQCSNPs was higher than that of I-131-TQ on SKOV3 cell lines.

引用

页码：71 / 85

页数：15

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