Metabolite profiling of two low phytic acid (lpa) rice mutants

被引：54

作者：

Frank, Thomas ^{[1
]}

Meuleye, Bertrand Seumo ^{[1
]}

Miller, Andreas ^{[1
]}

Shu, Qing-Yao ^{[2
]}

Engel, Karl-Heinz ^{[1
,2
]}

机构：

[1] Tech Univ Munich, Lehrstuhl Allgemeine Lebensmittechnol, D-85350 Freising Weihenstephan, Germany

[2] Zhejiang Univ, Inst Nucl Agr Sci, Key Lab Chinese Minist Agr Nucl Agr Sci, Hangzhou 310029, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2007年 / 55卷 / 26期

关键词：

low phytic acid mutants; rice; metabolite profiling; Oryza sativa L; phytic acid; myo-inositol; raffinose; myo-inositol hexakisphosphate; galactinol; myo-inositol kinase;

D O I：

10.1021/jf0723559

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Two low phytic acid (lpa) rice mutant lines, Os-lpa-XS110-1 and Os-lpa-XS110-2, were grown together with their parent wild-type variety Xiushui 110 in four field trials. HPLC analysis of inositol phosphates in the seeds produced demonstrated that compared to the wild-type, the reduction in phytic acid content in Os-lpa-XS110-1 (-46%) was more pronounced than that in Os-lpa-XS110-2 (-23%). Lower inositol phosphates (InsP(3), InsP(4), InsP(5)) were not detected in the mutants. The lpa mutants and the wild-type rice were subjected to comparative metabolite profiling by capillary gas chromatography. On average, 34% (Os-lpa-XS110-1) and 42% (Os-lpa-XS110-2) of the detected peaks were statistically significantly different between wild-type and mutants. However, only a few of these differences could be consistently observed for all field trials. Identification and quantification of the consistently different metabolites revealed that contents of myo-inositol and raffinose were increased in Os-lpa-XS110-1 but decreased in Os-lpa-XS110-2 compared to the wild-type. In addition, Os-lpa-XS110-1 exhibited increased levels of galactose and galactinol. Consideration of these metabolic changes in light of the routes involved in the biosynthesis of phytic acid indicated a disturbance in the early biosynthetic pathway of phytic acid in Os-lpa-XS110-2 (similar to the lpa-1 type mutation in maize) and a mutation event affecting phosphorylation of myo-inositol in Os-lpa-XS110-1 (similar to the lpa-3-type mutation).

引用

页码：11011 / 11019

页数：9

共 49 条

[1] Phosphorus and mineral concentrations in whole grain and milled low phytic acid (lpa) 1-1 rice
Bryant, RJ
Dorsch, JA
Peterson, KL
Rutger, JN
Raboy, V
[J]. CEREAL CHEMISTRY, 2005, 82 (05) : 517 - 522
[2] Unintended effects and their detection in genetically modified crops
Cellini, F
Chesson, A
Colquhoun, I
Constable, A
Davies, HV
Engel, KH
Gatehouse, AMR
Kärenlampi, S
Kok, EJ
Leguay, JJ
Lehesranta, S
Noteborn, HPJM
Pedersen, J
Smith, M
[J]. FOOD AND CHEMICAL TOXICOLOGY, 2004, 42 (07) : 1089 - 1125
[3] MICRODETERMINATION OF PHOSPHORUS
CHEN, PS
TORIBARA, TY
WARNER, H
[J]. ANALYTICAL CHEMISTRY, 1956, 28 (11) : 1756 - 1758
[4] Seed phosphorus and inositol phosphate phenotype of barley low phytic acid genotypes
Dorsch, JA
Cook, A
Young, KA
Anderson, JM
Bauman, AT
Volkmann, CJ
Murthy, PPN
Raboy, V
[J]. PHYTOCHEMISTRY, 2003, 62 (05) : 691 - 706
[5] Feng X., 2004, PLANT BIOTECHNOL, V21, P189, DOI DOI 10.5511/PLANTBIOTECHNOLOGY.21.183
[6] Metabolite profiling for plant functional genomics
Fiehn, O
Kopka, J
Dörmann, P
Altmann, T
Trethewey, RN
Willmitzer, L
[J]. NATURE BIOTECHNOLOGY, 2000, 18 (11) : 1157 - 1161
[7] Metabolomics - the link between genotypes and phenotypes
Fiehn, O
[J]. PLANT MOLECULAR BIOLOGY, 2002, 48 (1-2) : 155 - 171
[8] A methodology for automated comparative analysis of metabolite profiling data
Frenzel, T
Miller, A
Engel, KH
[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2003, 216 (04) : 335 - 342
[9] Metabolite profiling - A Fractionation method for analysis of major and minor compounds in rice grains
Frenzel, T
Miller, A
Engel, KH
[J]. CEREAL CHEMISTRY, 2002, 79 (02) : 215 - 221
[10] FRENZEL T, 2005, THESIS TU MUNICH GER

← 1 2 3 4 5 →