DNA N6-Adenine methylation in HBV-related hepatocellular carcinoma

被引：9

作者：

Cui, Hongyuan ^{[1
,6
]}

Rong, Weiqi ^{[2
]}

Ma, Jie ^{[6
]}

Zhu, Qing ^{[6
]}

Jiang, Boyue ^{[1
]}

Zhang, Lili ^{[4
]}

Li, Chang ^{[3
,4
]}

Zhuo, Zhongling ^{[3
,4
]}

Chen, Meng ^{[5
]}

机构：

[1] Beijing Hosp, Chinese Acad Med Sci, Inst Geriatr Med,Dept Gen Surg, Dept Hepatobilio Pancreat Surg,Natl Ctr Gerontol, Beijing 100730, Peoples R China

[2] Chinese Acad Med Sci & Peking Union Med Coll, Canc Hosp, Natl Clin Res Ctr Canc, Dept Hepatobiliary Surg,Natl Canc Ctr,Canc Hosp, Beijing, Peoples R China

[3] Beijing Hosp, Chinese Acad Med Sci, Beijing Inst Geriatr,Natl Ctr Gerontol, Inst Geriatr Med,Key Lab Geriatr,Natl Hlth Commis, Beijing, Peoples R China

[4] Beijing Hosp, Chinese Acad Med Sci, Inst Geriatr Med,Natl Hlth Commiss, Clin Biobank,Natl Ctr Gerontol,Natl Ctr Gerontol, Beijing, Peoples R China

[5] Chinese Acad Med Sci & Peking Union Med Coll, Canc Hosp, Natl Canc Data Ctr, Natl Canc Center,Nat Clin Res Ctr Canc, Beijing, Peoples R China

[6] Qinghai Univ, Dept Hepatopancreatobiliary Surg, Affiliated Hosp, Qinghai 810001, Peoples R China

来源：

GENE | 2022年 / 822卷

关键词：

6mA; Hepatocellular carcinoma; Nanopore sequencing; GENE-EXPRESSION; START SITES; CANCER; N-6-METHYLADENINE; N-6-ADENINE; PATTERNS; MOTIFS; ROLES;

D O I：

10.1016/j.gene.2022.146353

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

DNA methylation on N6-adenine (6mA) has recently been found to be a potential epigenetic marker in prokaryotes and eukaryotes. However, its distribution patterns and potential functions in human tumorigenesis remain largely unknown. Here, we reported global profiling of 6mA sites in the genome of hepatocellular carcinoma at single-nucleotide resolution using Nanopore sequencing. 6mA was widely distributed throughout the human genome. The 6mA sites were related to the porphyrin and chlorophyll metabolism in autosomes and were related to oxidative phosphorylation and ATP metabolism in mitochondria. AGG was the most significant motif associated with 6mA modification and the prevalent motifs in tumors were mainly distributed in mitochondria. The density of 6mA was related to the activation of gene transcription and 6mA density in repetitive sequences decreased in hepatocellular carcinoma. DNA 6mA methylation modification may also be a potential biomarker for cancer diagnosis and treatment.

引用

页数：9

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