The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor

被引：22

作者：

Chamsartra, S

Hewitt, CJ ^{[1
]}

Nienow, AW

机构：

[1] Univ Birmingham, Sch Engn Chem Engn, Ctr Formulat Engn, Birmingham B15 2TT, W Midlands, England

[2] Burapha Univ, Fac Sci, Dept Biol, BBERG, Chon Buri 20131, Thailand

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 10期

关键词：

agitated bioreactor; cell size; continuous cultivation; Corynebacterium glutamicum; flow cytometry; fluid mechanical stress;

D O I：

10.1007/s10529-005-4690-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of mechanical stresses generated by an extreme agitation intensity or a high aeration rate on growth parameters and cell physiology were studied during continuous cultivation of the Gram-positive bacterium Corynebacterium glutamicum. It is concluded that variations in agitation, aeration rate, or dO(2) concentrations down to about 1% of saturation do not damage the bacterial cells or cause a significant change in physiological response, as measured by flow cytometry, even though the cell size was slightly reduced.

引用

页码：693 / 700

页数：8

共 19 条

[1] The impact of fluid mechanical stress on Saccharomyces cerevisiae cells during continuous cultivation in an agitated, aerated bioreactor;: Its implication for mixing in the brewing process and aerobic fermentations [J].