Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

被引：1

作者：

Kang, Ji -Young ^{[1
]}

Mun, Dasom ^{[1
]}

Chun, Yumin ^{[1
]}

Park, Da -Seul ^{[1
]}

Kim, Hyoeun ^{[2
]}

Yun, Nuri ^{[3
]}

Lee, Seung-Hyun ^{[2
]}

Joung, Boyoung ^{[1
]}

机构：

[1] Yonsei Univ Coll Med, Div Cardiol, 50-1 Yonsei-ro, Seoul 03722, South Korea

[2] Yonsei Univ Coll Med, Dept Biochem & Mol Biol, 50-1 Yonsei-ro, Seoul 03722, South Korea

[3] Yonsei Univ, Inst Life Sci & Biotechnol, 50 Yonsei-ro, Seoul 03722, South Korea

来源：

STEM CELL RESEARCH | 2022年 / 64卷

基金：

新加坡国家研究基金会;

关键词：

TITIN;

D O I：

10.1016/j.scr.2022.102901

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

TTN mutations are the common genetic cause for various types of cardiomyopathies (e.g., dilated cardiomyopathy, hypertrophic cardiomyopathy, restrictive car-diomyopathy, and arrhythmogenic right ventricular cardiomyopathy) and skeletal myopathies. Here, we generated three TTN knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 system. These cell lines, which exhibit normal karyotype, typical morphology and pluripotency, could provide useful platform for investigating the role of TTN in associated disorders.

引用

页数：4

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