Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene

被引：21

作者：

Park, HS

Lee, JH

Jeong, EJ

Kim, JE

Hong, SJ

Park, TK

Kim, TY

Jang, WJ

Park, KH

Kim, BJ

Kook, YH

Lee, SH

机构：

[1] Konkuk Univ, Dept Microbiol, Coll Med, Chungju 380701, Chungchongbuk D, South Korea

[2] Konkuk Univ, Dept Pediat, Coll Med, Chungju 380701, Chungchongbuk D, South Korea

[3] Konkuk Univ, Dept Biotechnol, Chungju 380701, Chungchongbuk D, South Korea

[4] Minist Agr & Forestry, Div Anim Hlth, Kwacheon 427719, South Korea

[5] Seoul Natl Univ, Coll Med, Dept Microbiol, Seoul 110799, South Korea

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2005年 / 49卷 / 06期

关键词：

groEL gene; Rickettsia; duplex PCR;

D O I：

10.1111/j.1348-0421.2005.tb03760.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and the 213-bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.

引用

页码：545 / 549

页数：5

共 5 条

[1] Identification of Orientia tsutsugamushi, spotted fever group and typhus group Rickettsia by duplex and nested PCR methods
Luan, M-C
Yu, D-Z
Tang, L.
Zhang, L-J
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2008, 1 (04) : 1 - 8
[2] Identification of Orientia tsutsugamushi,spotted fever group and typhus group Rickettsia by duplex and nested PCR methods
M-C Luan
D-Z Yu
L Tang
L-J Zhang
Asian Pacific Journal of Tropical Medicine, 2008, 1 (04) : 1 - 8
[3] Rapid identification of goat (Capra hircus) and sheep (Ovis aries) species in raw meat using duplex PCR assay
Panwar, N.
Gahlot, G. C.
Gahlot, Kritika
Ashraf, M.
Singh, Anoop
INDIAN JOURNAL OF ANIMAL RESEARCH, 2015, 49 (04) : 537 - 541
[4] Identification of coagulase-negative staphylococci from bovine mastitis using RFLP-PCR of the groEL gene
da Silva Santos, Olinda Cabral
Barros, Elaine Menezes
Vasconcelos Paiva Brito, Maria Aparecida
de Freire Bastos, Maria do Carmo
Netto dos Santos, Katia Regina
Giambiagi-deMarval, Marcia
VETERINARY MICROBIOLOGY, 2008, 130 (1-2) : 134 - 140
[5] Diagnosis of mucormycosis using a simple duplex PCR assay: Analysis of 160 clinical samples from COVID-19 patients
Aboutalebian, Shima
Erami, Mahzad
Ahsaniarani, Amir Hossein
Momen-Heravi, Mansooreh
Sharif, Alireza
Hadipour, Mahboubeh
Mirhendi, Hossein
MEDICAL MYCOLOGY, 2023, 61 (09)

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