Isolation of microsatellite loci in the codling moth, Cydia pomonella (Lepidoptera: Tortricidae)

被引：16

作者：

Zhou, YH ^{[1
]}

Gu, HN ^{[1
]}

Dorn, S ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Inst Plant Sci Appl Entomol, CH-8092 Zurich, Switzerland

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 02期

关键词：

codling moth; Cydia pomonella; Lepidoptera; microsatellite;

D O I：

10.1111/j.1471-8286.2004.00886.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cydia pomonella L. is an important insect pest of pome fruits worldwide. We have isolated and characterized 17 microsatellite loci from the enriched genomic libraries constructed using a biotin/streptavidin capture protocol. Among these loci, 11 were scored polymorphic when 50 individuals from a laboratory population were screened. Their alleles numbered two to four, with the observed heterozygosity (H-O) ranging from 0.114 to 0.404. Successful amplification was obtained for all these loci when the designed primers were tested, showing the promise of use in genetic mapping and population studies.

引用

页码：226 / 227

页数：2

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