Report on the first detection of pectenotoxin-2, spirolide-A and their derivatives in French shellfish

被引：51

作者：

Amzil, Zouher ^{[1
]}

Sibat, Manoella ^{[1
]}

Royer, Florence ^{[1
]}

Masson, Nadine ^{[2
]}

Abadie, Eric ^{[3
]}

机构：

[1] IFREMER, Microbiol & Phycotoxins Dept, F-44311 Nantes, France

[2] IFREMER, Stn Arcachon, F-33120 Arcachon, France

[3] IFREMER, Stn Sete, Sete, France

来源：

MARINE DRUGS | 2007年 / 5卷 / 04期

关键词：

DSP toxins; spirolides; pectenotoxins; LC/MS analysis;

D O I：

10.3390/md504168

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

In the context of the French Phytoplankton and Phycotoxins Monitoring Network (REPHY) programme, shellfish samples were harvested from different locations where harmful algae blooms were known to have occurred. For all shellfish samples found positive by the mouse bioassay for diarrhetic shellfish poisoning (DSP) toxins, liquid chromatography (LC) coupled with mass spectrometry (MS) was used to search for the following lipophilic toxins: okadaic acid (OA), dinophysistoxins (DTXs), pectenotoxins (PTXs), azaspiracids (AZAs), yessotoxins (YTXs), spirolides (SPXs) and gymnodimines (GYMs). In order to investigate the presence of acyl-OAs and/or acyl-DTX-1,-2 (DTX-3), alkaline hydrolysis was performed on all samples, and LC/ MS analyses were carried out on the samples before and after hydrolysis. The results revealed different lipophilic toxin profiles as a function of the shellfish sampling location. The primary finding was that all of the samples contained OA and acyl-OA. In addition, other lipophilic toxins were found in shellfish samples: DTX-2, acyl-DTX-2 and SPXs (SPX-A, SPX-desMeC) on the Atlantic coast (Southern Brittany, Arcachon), and pectenotoxins (PTX-2, PTX-2-seco-acid and 7-epi-PTX-2-seco-acid) on the Mediterranean coast (Thau lagoon, the island of Corsica). This paper reports on the first detection of PTX-2, SPX-A and their derivatives in French shellfish.

引用

页码：168 / 179

页数：12

共 36 条

[1] Detection and identification of spirolides in Norwegian shellfish and plankton [J].

Aasen, J ;

MacKinnon, SL ;

LeBlanc, P ;

Walter, JA ;

Hovgaard, P ;

Aune, T ;

Quilliam, MA .

CHEMICAL RESEARCH IN TOXICOLOGY, 2005, 18 (03) :509-515

[2] Domoic acid accumulation in French shellfish in relation to toxic species of Pseudo-nitzschia multiseries and P-pseudodelicatissima [J].

Amzil, Z ;

Fresnel, J ;

Le Gal, D ;

Billard, C .

TOXICON, 2001, 39 (08) :1245-1251

[3]

Amzil Zouher, 2006, P150

[4]

Biré R, 2002, J NAT TOXINS, V11, P269

[5] Identification of pectenotoxins in plankton, filter feeders, and isolated cells of a Dinophysis acuminata with an atypical toxin profile, from Chile [J].

Blanco, Juan ;

Alvarez, Gonzalo ;

Uribe, Eduardo .

TOXICON, 2007, 49 (05) :710-716

[6]

Cembella A., 1998, Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO., P481

[7] The marine dinoflagellate Alexandrium ostenfeldii (Dinophyceae) as the causative organism of spirolide shellfish toxins [J].

Cembella, AD ;

Lewis, NI ;

Quilliam, MA .

PHYCOLOGIA, 2000, 39 (01) :67-74

[8] Toxin profile of Alexandrium ostenfeldii (Dinophyceae) from the Northern Adriatic Sea revealed by liquid chromatography-mass spectrometry [J].

Ciminiello, P ;

Dell'Aversano, C ;

Fattorusso, E ;

Magno, S ;

Tartaglione, L ;

Cangini, M ;

Pompei, M ;

Guerrini, F ;

Boni, L ;

Pistocchi, R .

TOXICON, 2006, 47 (05) :597-604

[9] ISOLATION OF A NEW DIARRHETIC SHELLFISH POISON FROM IRISH MUSSELS [J].

HU, TM ;

DOYLE, J ;

JACKSON, D ;

MARR, J ;

NIXON, E ;

PLEASANCE, S ;

QUILLIAM, MA ;

WALTER, JA ;

WRIGHT, JLC .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1992, (01) :39-41

[10] SPIROLIDE-B AND SPIROLIDE-D, 2 NOVEL MACROCYCLES ISOLATED FROM THE DIGESTIVE GLANDS OF SHELLFISH [J].

HU, TM ;

CURTIS, JM ;

OSHIMA, Y ;

QUILLIAM, MA ;

WALTER, JA ;

WATSONWRIGHT, WM ;

WRIGHT, JLC .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1995, (20) :2159-2161

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