Factors influencing Agrobacterium tumefaciens-mediated genetic transformation of Eleusine coracana (L.) Gaertn

被引：39
作者：
Sharma, Manju [3 ]
Kothari-Chajer, Aditi [1 ]
Jagga-Chugh, Swati [2 ]
Kothari, S. L. [1 ,2 ]
机构：
[1] Univ Rajasthan, Dept Bot, Expt Morphogenesis & Plant Tissue Culture Lab, Jaipur 302004, Rajasthan, India
[2] Univ Rajasthan, Ctr Converging Technol, Jaipur 302004, Rajasthan, India
[3] Jimma Univ, Dept Hort & Plant Sci, Coll Agr & Vet Med, Jimma, Ethiopia
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2011年 / 105卷 / 01期
关键词：
Finger millet; Plant regeneration; Transgenic plants; GUS; Ragi; npt II; PLANT-REGENERATION; T-DNA; EMBRYOGENIC CALLUS; MATURE EMBRYO; RICE; EXPRESSION; EXPLANTS;
D O I：
10.1007/s11240-010-9846-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Agrobacterium-mediated transformation protocol has been developed for Eleusine coracana (var. PR-202) by varying several factors which influence T-DNA delivery. Green nodular regenerative calli with meristematic nodules of seed origin were used as the target tissue for Agrobacterium tumefaciens-mediated gene transfer. The highest frequency of transformation (44.4%) was observed when callus was infected, co-cultivated and incubated at 22A degrees C. Incorporation of higher level of CuSO4 in the regeneration medium had significantly positive effect on the recovery of transformed plants. PCR analysis of T (0) and T (1) generation plants with nptII-specific primers revealed the amplification of nptII gene. Southern blot analysis of six regenerated plants confirmed selectable marker gene integration in three plants. This is a first report on Agrobacterium-mediated genetic transformation of finger millet and will pave the way for further studies in this and other millet crops.
引用
收藏
页码：93 / 104
页数：12
相关论文
共 54 条
  • [1] Ali S., 2007, Biotechnology, V6, P138
  • [2] Altpeter F., 2005, International Turfgrass Society Research Journal, V10, P485
  • [3] Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue
    Amoah, BK
    Wu, H
    Sparks, C
    Jones, HD
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 2001, 52 (358) : 1135 - 1142
  • [4] [Anonymous], 2001, Plant Biotechnol, DOI DOI 10.5511/PLANTBIOTECHNOLOGY.18.275
  • [5] An efficient protocol for sugarcane (Saccharum spp. L.) transformation mediated by Agrobacterium tumefaciens
    Arencibia, AD
    Carmona, ER
    Tellez, P
    Chan, MT
    Yu, SM
    Trujillo, LE
    Oramas, P
    [J]. TRANSGENIC RESEARCH, 1998, 7 (03) : 213 - 222
  • [6] Armstrong CL, 2001, Int Patent Publ, Patent No. [WOO1/09302 A2, 0109302, 109302]
  • [7] Bechtold N, 2000, GENETICS, V155, P1875
  • [8] Plant regeneration through callus initiation from mature embryo of Triticum
    Bi, R. M.
    Kou, M.
    Chen, L. G.
    Mao, S. R.
    Wang, H. G.
    [J]. PLANT BREEDING, 2007, 126 (01) : 9 - 12
  • [9] High frequency Agrobacterium tumefaciens-mediated plant transformation induced by ammonium nitrate
    Boyko, Alex
    Matsuoka, Aki
    Kovalchuk, Igor
    [J]. PLANT CELL REPORTS, 2009, 28 (05) : 737 - 757
  • [10] Genetic engineering of millets: current status and future prospects
    Ceasar, S. Antony
    Ignacimuthu, S.
    [J]. BIOTECHNOLOGY LETTERS, 2009, 31 (06) : 779 - 788
123456