Sensitivity enhancement in the TROSY experiment

被引：88

作者：

Czisch, M ^{[1
]}

Boelens, R ^{[1
]}

机构：

[1] Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3584 CH Utrecht, Netherlands

来源：

JOURNAL OF MAGNETIC RESONANCE | 1998年 / 134卷 / 01期

关键词：

HSQC; N-15; NMR; protein NMR; serine protease; gradient spectroscopy;

D O I：

10.1006/jmre.1998.1483

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modification of the TROSY experiment which allows for sensitivity enhancement without introduction of additional delays is presented. The method relies on the combination of two orthogonal pathways and results in a signal-to-noise gain of root 2 independent of the size of the molecule. The sensitivity enhanced TROSY scheme can be combined with gradient coherence selection. The method has been applied to the 269 residue serine protease PB92. (C) 1998 Academic Press.

引用

页码：158 / 160

页数：3

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