Real-time and long-time in vivo imaging in the shortwave infrared window of perforator vessels for more precise evaluation of flap perfusion

被引：26

作者：

Feng, Shaoqing ^{[1
]}

Chen, Jun ^{[2
]}

Wo, Yan ^{[1
,3
]}

Li, Yunxia ^{[2
]}

Chen, Shiyi ^{[2
]}

Zhang, Yixin ^{[1
]}

Zhang, Wenjie ^{[1
,4
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Sch Med, Shanghai 200011, Peoples R China

[2] Fudan Univ, Huashan Hosp, Dept Orthoped Sports Med, Shanghai 200040, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Med, Dept Human Anat Histol & Embryol, Shanghai 200025, Peoples R China

[4] Natl Tissue Engn Ctr China, Shanghai Key Lab Tissue Engn, Shanghai 200011, Peoples R China

来源：

BIOMATERIALS | 2016年 / 103卷

基金：

中国博士后科学基金; 上海市自然科学基金; 中国国家自然科学基金;

关键词：

Imaging agents; Quantum dots; Shortwave infrared region; Flap perfusion; Intraoperative assessment; SULFIDE QUANTUM DOTS; 1.3; MU-M; ELECTRONIC-STRUCTURE; PBSE; FLUORESCENCE; VISUALIZATION;

D O I：

10.1016/j.biomaterials.2016.06.043

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Effective real-time and long-time in vivo imaging for flap perfusion requires bright and stable imaging agents whose emissions can effectively penetrate live tissues without photobleaching. Compared to the standard imaging agent today - intraoperative indocyanine green (ICG), quantum dots (QDs) is a more attractive alternative due to its excellent optical properties including broad emission spectrum and stability against photobleaching. Recent studies have confirmed that the shortwave infrared window (SWIR) between 1000 and 2300 nm is the most sensitive spectral range for in vivo imaging due to its extremely low tissue absorption and autofluorescence. Here, we, for the first time, report a novel approach of flap perfusion assessment that provides real-time and long-time in vivo imaging using lead sulfide (PbS) QDs. Our results show that PbS QDs, as an imaging agent, can improve the stability of in vivo high-resolution images in a sustained manner, thus facilitating the precise evaluation of flap perfusion. In summary, compared to current imaging reporters, SWIR QDs have high photostability and deep tissue penetration, which makes them as promising in vivo imaging agents for more precise evaluation of flap perfusion. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：256 / 264

页数：9

共 38 条

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