Green synthesis, characterization and optimization of silver nanoparticles using honey and antimicrobial study with food supplements

Nanotechnology is an emerging field with a vast role in health, cosmetics and industrial applications worldwide. Nanoparticles of silver varying from 1 to 1000 nm are referred to as silver nanoparticles. In the current study, an attempt has been made for the first time to synthesize silver nanoparticles from two varieties of honey available in sultanate of Oman. The existence of silver nanoparticles was identified by UV visible spectroscopy, which showed a characteristic peak at 450 nm. By varying concentration of silver nitrate, honey, pH and temperature, optimization studies for synthesis of nanoparticles were carried out. These parameters play a vital role not only in synthesizing silver particles, but also help to stabilize the nanoparticles. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM), studies were carried out on the nanoparticles to understand their shape, size, structure and optical properties. Antimicrobial studies of silver nanoparticles with honey individually and synergistic effect in combination with food supplements showed significant zone of inhibition against gram-positive and gram-negative bacteria. These findings revealed that honey-based nanoparticles with food supplements could be used effectively to control common diseases.