Heterologous expression of sarcoplasmic reticulum Ca2+-ATPase

被引：5
作者：
Reis, EMR [1 ]
Slayman, CW [1 ]
VerjovskiAlmeida, S [1 ]
机构：
[1] YALE UNIV,SCH MED,DEPT GENET,NEW HAVEN,CT 06510
来源：
BIOSCIENCE REPORTS | 1996年 / 16卷 / 02期
关键词：
ATPase; calcium; expression systems; sarcoplasmic reticulum;
D O I：
10.1007/BF01206200
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In recent years, expression of rabbit sarcoplasmic reticulum (SR) Ca2+-ATPase in heterologous systems has been a widely used strategy to study altered enzymes generated by site-directed mutagenesis. Various eukaryotic expression systems have been tested, all of them yielding comparable amounts of recombinant protein. However, the relatively low yield of recombinant protein obtained so far suggests that novel purification techniques will be required to allow further characterization of this enzyme based on direct ligand-binding measurements.
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收藏
页码：107 / 113
页数：7
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