Cumulative pregnancy rate after ICSI with cryopreserved testicular tissue in non-obstructive azoospermia

被引：24

作者：

Dafopoulos, K ^{[1
]}

Griesinger, G ^{[1
]}

Schultze-Mosgau, A ^{[1
]}

Orief, Y ^{[1
]}

Schöpper, B ^{[1
]}

Nikolettos, N ^{[1
]}

Diedrich, K ^{[1
]}

Al-Hasani, S ^{[1
]}

机构：

[1] Univ Schleswig Holstein, Dept Obstet & Gynaecol, D-23538 Lubeck, Germany

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2005年 / 10卷 / 04期

关键词：

D O I：

10.1016/S1472-6483(10)60821-8

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The aim of the present study was to describe a simplified and inexpensive method of testicular tissue freezing to assess the cumulative clinical pregnancy rate (CRR) by this technique and to provide useful information for counselling couples with non-obstructive azoospermia. One hundred and sixty-five couples with non-obstructive azoospermic males pursuing assisted conception, from December 2002, were included. In all cases, the testicular tissue retrieved by open multiple-biopsy (both sides, by testicular sperm extraction) was frozen using a simple liquid nitrogen vapour freezing technique and was stored in liquid nitrogen thereafter. Only mature spermatozoa were used for intracyloplasmic sperm injection (ICSI) after thawing. Expected CRR were calculated using the Kapian-Meter survival analysis. A total of 289 cycles were performed resulting in 53 clinical pregnancies. Crude and expected CPR (95% confidence intervals) after three cycles were 32.1 (25.7-40.1) and 55.7% (37.0-74.4) respectively. In conclusion, this simplified method for freezing testicular tissue resulted in a satisfactory outcome after ICSI in cases of non-obstructive azoospermia.

引用

页码：461 / 466

页数：6

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