Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis

被引：30
作者：
Xu, Ruodan [1 ]
Zhao, Huiling [2 ]
Muhammad, Hanif [2 ]
Dong, Mingdong [2 ]
Besenbacher, Flemming [2 ]
Chen, Menglin [1 ,2 ]
机构：
[1] Aarhus Univ, Dept Engn, DK-8000 Aarhus, Denmark
[2] Aarhus Univ, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus C, Denmark
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词：
MESENCHYMAL STEM-CELLS; SONIC HEDGEHOG; STROMAL CELLS; DIFFERENTIATION; GROWTH; NANOFIBERS; FABRICATION; FGF2; OSTEOGENESIS; COMBINATION;
D O I：
10.1038/s41598-017-08226-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly(L-lactic acid-co-epsilon-caprolactone)-polyethylene oxide (SF/PLCL-PEO) enhanced fibrogenic lineage differentiation of MSCs. The core-shell structure was confirmed by transmission electron microscopy (TEM), fluorescence microscopy and attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy. A sequential release of FGF-2 and CTGF was successfully achieved in this manner. FGF-2 plays an important role in stem cell proliferation and, meanwhile when accompanied with CTGF, has a slightly additive effect on fibrogenic differentiation of MSCs, whereas CTGF promotes fibrogenesis and alleviates osteogenesis, chondrogenesis and adipogenesis.
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页数：11
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