Are the mitochondrial respiratory complexes blocked by NO the targets for the laser and LED therapy?

被引：17

作者：

Buravlev, Evgeny A. ^{[1
]}

Zhidkova, Tatyana V. ^{[2
]}

Osipov, Anatoly N. ^{[1
]}

Vladimirov, Yury A. ^{[2
]}

机构：

[1] NI Pirogov Russian Natl Res Med Univ, Dept Gen & Med Biophys, Moscow 117997, Russia

[2] Moscow MV Lomonosov State Univ, Fac Fundamental Med, Moscow 119192, Russia

来源：

LASERS IN MEDICAL SCIENCE | 2015年 / 30卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

Nitric oxide; Mitochondria; Low-level laser therapy; Mitochondrial electron transport chain; CYTOCHROME-C-OXIDASE; NITRIC-OXIDE; ENDOTOXIC-SHOCK; VISIBLE-LIGHT; IRRADIATION; CELLS; LIVER; INHIBITION; HEMOGLOBIN; RADIATION;

D O I：

10.1007/s10103-014-1639-8

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Effects of laser (442 and 532 nm) and light-emitting diode (LED) (650 nm) radiation on mitochondrial respiration and mitochondrial electron transport rate (complexes II-III and IV) in the presence of nitric oxide (NO) were investigated. It was found that nitric oxide (300 nM-10 mu M) suppresses mitochondrial respiration. Laser irradiation of mitochondria (442 nm, 3 J cm(-2)) partly restored mitochondrial respiration (approximately by 70 %). Irradiation with green laser (532 nm) or red LED (650 nm) in the same dose had no reliable effect. Evaluation of mitochondrial electron transport rate in complexes II-III and IV and effects of nitric oxide demonstrated almost similar sensitivity of complex II-III and IV to NO, with approximately 50 % inhibition at NO concentration of 3 mu M. Subsequent laser or LED irradiation (3 J cm(-2)) showed partial recovery of electron transport only in complex IV and only under irradiation with blue light (442 nm). Our results support the hypothesis of the crucial role of cytochrome c oxidase (complex IV) in photoreactivation of mitochondrial respiration suppressed by NO.

引用

页码：173 / 180

页数：8

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