Angiotensin-Converting Enzyme 2 (ACE2) As a Novel Biorecognition Element in A Cell-Based Biosensor for the Ultra-Rapid, Ultra-Sensitive Detection of the SARS-CoV-2 S1 Spike Protein Antigen

被引：7

作者：

Mavrikou, Sofia ^{[1
]}

Tsekouras, Vasileios ^{[1
]}

Hatziagapiou, Kyriaki ^{[2
,3
,4
]}

Tsalidou, Asimina ^{[1
]}

Bakakos, Petros ^{[5
]}

Rovina, Nikoletta ^{[5
]}

Koutsoukou, Antonia ^{[5
]}

Michos, Athanasios ^{[2
]}

Nikola, Olti ^{[2
]}

Koniari, Eleni ^{[6
,7
]}

Papaparaskevas, Joseph ^{[8
]}

Chrousos, George P. ^{[6
,7
]}

Kanaka-Gantenbein, Christina ^{[2
]}

Kintzios, Spyridon ^{[1
]}

机构：

[1] EU CONEXUS European Univ, Agr Univ Athens, Dept Biotechnol, Lab Cell Technol, Athens 11855, Greece

[2] Natl & Kapodistrian Univ Athens, Agia Sofia Childrens Hosp, Dept Pediat 1, Thivon 1, Athens 11527, Greece

[3] State Univ West Attica, Fac Hlth & Care Sci, Physiotherapy Dept, Agiou Spiridonos 28, Athens 12243, Greece

[4] State Univ West Attica, Fac Hlth & Care Sci, Dept Nursing, Agiou Spiridonos 28, Athens 12243, Greece

[5] Natl & Kapodistrian Univ Athens, Sotiria Hosp, Med Sch, Univ Dept Resp Med 1, 152 Mesogeion Ave, Athens 11527, Greece

[6] Natl & Kapodistrian Univ Athens, Agia Sofia Childrens Hosp, Univ Res Inst Maternal & Child Hlth & Precis Med, Thivon & Livadias 8 Str, Athens 11527, Greece

[7] Natl & Kapodistrian Univ Athens, Agia Sofia Childrens Hosp, UNESCO Chair Adolescent Hlth Care, Thivon & Livadias 8 Str, Athens 11527, Greece

[8] Natl & Kapodistrian Univ Athens, Med Sch, Dept Microbiol, Mikras Asias 75, Athens 11527, Greece

来源：

CHEMOSENSORS | 2021年 / 9卷 / 12期

关键词：

bioelectric recognition assay (BERA); membrane engineering; public health surveillance; S1 spike protein; rapid antigen test; serological assay; severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2); BIOELECTRIC RECOGNITION ASSAY; RECEPTOR; EXPRESSION; HOMOLOG; BINDING; TARGET; MODEL;

D O I：

10.3390/chemosensors9120341

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Antigen screening for the SARS-CoV-2 S1 spike protein is among the most promising tools for the mass monitoring of asymptomatic carriers of the virus, especially in limited resource environments. Herewith, we report on the possible use of the angiotensin-converting enzyme 2 (ACE2), the natural receptor and entry point of the virus, as a biorecognition element for the detection of the S1 antigen combined with an established bioelectric biosensor based on membrane-engineered cells. The working principle of our approach is based on the measurable change of the electric potential of membrane-engineered mammalian cells bearing ACE2 after attachment of the respective viral protein. We demonstrate that sensitive and selective detection of the S1 antigen is feasible in just three min, with a limit of detection of 20 fg/mL. In a preliminary clinical application, positive patient-derived samples were identified with a 87.9% score compared to RT-PCR. No cross-reactivity was observed against a wide range of nucleocapsid protein concentrations. The novel biosensor is embedded in a commercially ready-to-use testing platform, complete with the consumable immobilized cell-electrode interface and a portable read-out device operable through smartphone or tablet. In addition, the possible application of the system for the high throughput screening of potential pharmacological inhibitors of the ACE2 receptor-S1 RBD interaction is discussed.

引用

页数：13

共 45 条

[1] Newly Developed System for Acetamiprid Residue Screening in the Lettuce Samples Based on a Bioelectric Cell Biosensor [J].