Effects of prolonged delivery of brain-derived neurotrophic factor on the fate of neural stem cells transplanted into the developing rat retina

被引：33

作者：

Suzuki, T ^{[1
]}

Ooto, S ^{[1
]}

Akagi, T ^{[1
]}

Amemiya, K ^{[1
]}

Igarashi, R ^{[1
]}

Mizushima, Y ^{[1
]}

Takahashi, M ^{[1
]}

机构：

[1] Kyoto Univ Hosp, Translat Res Ctr, Dept Expt Therapeut, Kyoto 6068507, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 309卷 / 04期

关键词：

neural stem cell; stem cell transplantation; retina; neurotrophins; brain-derived neurotrophic factor; delivery; differentiation;

D O I：

10.1016/j.bbrc.2003.08.076

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the major roles of brain-derived neurotrophic factor (BDNF) is to promote the differentiation and support the survival of neurons in the central nervous system. The objective of the present study was to evaluate the effect of BDNF on the fate of adult rat hippocampus-derived neural stem cells (AHPCs) transplanted into the developing rat retina. Immunohistochemical analysis showed a significant increase in the ratio of grafted AHPCs stained for MAP2ab (P < 0.05) and a marked decrease in the ratio of nestin-positive grafted cells in the slow-releasing BDNF group compared with the control group. The respective changes in the ratios of MAP5 and GFAP-positive grafted cells were comparable for the two groups. The results reported here suggest a potentially beneficial role for extended delivery of BDNF in the differentiation of grafted neural stem cells, which may lead to a novel modification of stem cell transplantation. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：843 / 847

页数：5

共 28 条

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