Measuring the transmission matrix of a scattering medium using epi-fluorescence light

被引:2
作者
Premillieu, Evolene [1 ]
Piestun, Rafael [1 ]
机构
[1] Univ Colorado, Dept Elect Comp & Energy Engn, Boulder, CO 80309 USA
基金
美国国家科学基金会;
关键词
MICROSCOPY;
D O I
10.1016/j.optcom.2019.125207
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
There are several methods to focus light behind a scattering medium, but very few use fluorescence light as feedback and can be used without access to the distal side of the scatterer. Among all the wave-front shaping techniques, retrieving the transmission matrix of a scattering material is the only one that allows for focusing on multiple spots after a single set of measurements. Here we propose a method to retrieve the transmission matrix of a scatterer using fluorescence light as feedback without access to the distal side. The advantage of this method is that it allows focusing on the whole field of view after a single matrix measurement without access to the distal side of the sample, making it suitable for reflection epi-illumination geometry microscopy.
引用
收藏
页数:5
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