Deletion of two-component system QseBC weakened virulence of Glaesserella parasuis in a murine acute infection model and adhesion to host cells

被引：9
作者：
Yan, Xuefeng [1 ]
Dai, Ke [2 ]
Gu, Congwei [1 ]
Yu, Zehui [1 ]
He, Manli [1 ]
Xiao, Wudian [1 ]
Zhao, Mingde [1 ]
He, Lvqin [1 ]
机构：
[1] Southwest Med Univ, Luzhou, Peoples R China
[2] Sichuan Agr Univ, Chengdu, Peoples R China
来源：
PEERJ | 2022年 / 10卷
关键词：
Glaesserella parasuis; TCS; Quorum sensing; QseBC; Adherence; Virulence; HAEMOPHILUS-PARASUIS; BIOFILM FORMATION; STRESS RESISTANCE; SERUM RESISTANCE; COLONIZATION; REGULATOR; BACTERIA;
D O I：
10.7717/peerj.13648
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The widespread two-component system (TCS), QseBC, involves vital virulence regulators in Enterobacteriaceae and Pasteurellaceae. Here we studied the function of QseBC in Glaesserella parasuis. A AqseBC mutant was constructed using a Glaesserella parasuis serovar 11 clinical strain SC1401 by natural transformation. Immunofluorescence was used to evaluate cellular adhesion, the levels of inflammation and apoptosis. The ability of AqseBC and AqseC mutant strains to adhere to PAM and MLE-12 cells was significantly reduced. Additionally, by focusing on the clinical signs, H&E, and IFA for inflammation and apoptosis, we found that the AqseBC mutant weakened virulence in the murine models. Together, these findings suggest that QseBC plays an important role in the virulence of Glaesserella parasuis.
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页数：22
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