Pregnancy achieved by transfer of a single blastocyst selected by time-lapse monitoring

被引：85

作者：

Pribenszky, Csaba ^{[1
]}

Matyas, Szabolcs ^{[2
]}

Kovacs, Peter ^{[2
]}

Losonczi, Eszter ^{[3
]}

Zadori, Janos ^{[4
]}

Vajta, Gabor ^{[5
]}

机构：

[1] Szent Istvan Univ, Dept Anim Breeding & Genet, Fac Vet Sci, H-1078 Budapest, Hungary

[2] KAALI Inst Budapest, H-1125 Budapest, Hungary

[3] ARTech Co Res & Dev, Debrecen, Hungary

[4] Univ Szeged, Ctr Assisted Reprod, Kaali Inst, H-6725 Budapest, Hungary

[5] Beijing Genom Inst Shenzhen, Shenzhen, Peoples R China

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2010年 / 21卷 / 04期

关键词：

early cleavage; fragmentation; single-embryo transfer; time-lapse monitoring; EMBRYO DEVELOPMENT; IN-VITRO; FRAGMENTATION; FERTILIZATION; EMBRYOGENESIS; FIELDS;

D O I：

10.1016/j.rbmo.2010.04.015

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Appropriate selection of a single blastocyst for transfer decreases the risk of multiple gestations. By using a compact time-lapse microscope system placed inside a regular incubator, combined with a microwell embryo culture dish, the development of all the embryos from a patient was continuously monitored by obtaining images at 10 min intervals. The embryos were not moved during the time-lapse observation. The system was switched off completely between image acquisitions in order to avoid exposure to electromagnetic radiation. The analysis of time-lapse records was used to choose a single blastocyst for transfer, which resulted in a singleton pregnancy and birth of a healthy boy on term. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

引用

页码：533 / 536

页数：4

共 14 条

[1] Mouse early embryos obtained by natural breeding or in vitro fertilization display a differential sensitivity to extremely low-frequency electromagnetic fields [J].

Beraldi, R ;

Sciamanna, I ;

Mangiacasale, R ;

Lorenzini, R ;

Spadafora, C .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2003, 538 (1-2) :163-170

[2] ENVIRONMENTAL MAGNETIC-FIELDS - INFLUENCES ON EARLY EMBRYOGENESIS [J].

CAMERON, IL ;

HARDMAN, WE ;

WINTERS, WD ;

ZIMMERMAN, S ;

ZIMMERMAN, AM .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, 51 (04) :417-425

[3]

GONZALES DS, 1995, J REPROD FERTIL, V105, P1, DOI 10.1530/jrf.0.1050001

[4] Cinematographs of living developing rabbit-eggs [J].

Gregory, PW .

SCIENCE, 1929, 69 :226-229

[5] DETECTION OF FERTILIZATION IN EMBRYOS WITH ACCELERATED CLEAVAGE BY FLUORESCENT IN-SITU HYBRIDIZATION (FISH) [J].

HARPER, JC ;

ROBINSON, F ;

DUFFY, S ;

GRIFFIN, DK ;

HANDYSIDE, AH ;

DELHANTY, JDA ;

WINSTON, RML .

HUMAN REPRODUCTION, 1994, 9 (09) :1733-1737

[6] Porcine embryo development and fragmentation and their relation to apoptotic markers: a cinematographic and confocal laser scanning microscopic study [J].

Mateusen, B ;

Van Soom, A ;

Maes, DGD ;

Donnay, I ;

Duchateau, L ;

Lequarre, AS .

REPRODUCTION, 2005, 129 (04) :443-452

[7] TIMING OF DEVELOPMENT IS A CRITICAL PARAMETER FOR PREDICTING SUCCESSFUL EMBRYOGENESIS [J].

MCKIERNAN, SH ;

BAVISTER, BD .

HUMAN REPRODUCTION, 1994, 9 (11) :2123-2129

[8] Metabolomic assessment of oocyte viability [J].

Nagy, Zsolt Peter ;

Jones-Colon, Stacey ;

Roos, Pieter ;

Botros, Lucy ;

Greco, Ermanno ;

Dasig, Jennifer ;

Behr, Barry .

REPRODUCTIVE BIOMEDICINE ONLINE, 2009, 18 (02) :219-225

[9] IVF/ICSI twin pregnancies: risks and prevention [J].

Pinborg, A .

HUMAN REPRODUCTION UPDATE, 2005, 11 (06) :575-593

[10] Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment [J].

Pribenszky, Csaba ;

Losonczi, Eszter ;

Molnar, Miklos ;

Lang, Zsolt ;

Matyas, Szabolcs ;

Rajczy, Klara ;

Molnar, Katalin ;

Kovacs, Peter ;

Nagy, Peter ;

Conceicao, Jason ;

Vajta, Gabor .

REPRODUCTIVE BIOMEDICINE ONLINE, 2010, 20 (03) :371-379

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