X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads

被引:16
作者
Chen, Zhen-Xia [1 ]
Oliver, Brian [1 ]
机构
[1] NIDDK, Sect Dev Gen, Lab Cellular & Dev Biol, NIH, Bethesda, MD 20892 USA
来源
G3-GENES GENOMES GENETICS | 2015年 / 5卷 / 06期
基金
美国国家卫生研究院;
关键词
segmental aneuploidy; sex determination; dosage compensation; sex chromosomes; gene expression; genetics of sex; RNA-SEQ; LINKED GENES; COPY NUMBER; COMPENSATION; EXPRESSION; EVOLUTION; ANEUPLOIDY; ALIGNMENT;
D O I
10.1534/g3.115.017632
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
X chromosome dosage compensation is required for male viability in Drosophila. Dosage compensation relative to autosomes is two-fold, but this is likely to be due to a combination of homeostatic gene-by-gene regulation and chromosome-wide regulation. We have baseline values for gene-by-gene dosage compensation on autosomes, but not for the X chromosome. Given the evolutionary history of sex chromosomes, these baseline values could differ. We used a series of deficiencies on the X and autosomes, along with mutations in the sex-determination gene transformer-2, to carefully measure the sex-independent X-chromosome response to gene dosage in adult heads by RNA sequencing. We observed modest and indistinguishable dosage compensation for both X chromosome and autosome genes, suggesting that the X chromosome is neither inherently more robust nor sensitive to dosage change.
引用
收藏
页码:1057 / 1063
页数:7
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