The Polymerization of Aeromonas hydrophila AH-3 O-Antigen LPS: Concerted Action of WecP and Wzy

被引：4
作者：
Merino, Susana [1 ]
Gonzalez, Victor [1 ]
Tomas, Juan M. [1 ]
机构：
[1] Univ Barcelona, Fac Biol, Dept Microbiol, Barcelona 08071, Spain
来源：
PLOS ONE | 2015年 / 10卷 / 07期
关键词：
LIPOPOLYSACCHARIDE; POLYSACCHARIDE; TRANSLOCATION; BIOSYNTHESIS;
D O I：
10.1371/journal.pone.0131905
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The repeat units of heteropolymeric O antigen are synthesized at the cytosolic side of the inner bacterial membrane via the Wzx/Wzy-dependent assembly pathway. After being translocated across the membrane by Wzx, each repeat unit is polymerized by Wzy to form a glycan chain. In this study, we demonstrate the need of the corresponding enzyme transferring the initial HexNAc to undecaprenol phosphate (lipid carrier), together with the corresponding O-antigen polymerase (Wzy), to produce the Aeromonas hydrophila O:34-antigen. We suggest, the concerted action of WecA or P enzyme (UDP-HexNAc: polyprenol-P HexNAc-1-P transferase) and Wzy is involved in the mechanism responsible for the A. hydrophila O-antigen polymerization.
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页数：15
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