Quantification of the orientations of pyrrolidine-based oxypeptide nucleic acid-DNA hybrid duplexes

被引：0

作者：

Kitamatsu, Mizuki ^{[1
]}

Sisido, Masahiko ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Nat Sci & Technol, Dept Med & Bioengn, Kita Ku, Okayama 7000082, Japan

来源：

MEDCHEMCOMM | 2011年 / 2卷 / 07期

关键词：

HYBRIDIZATION; BINDING; SPECIFICITY; ANALOGS;

D O I：

10.1039/c0md00223b

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the fluorescence quenching-based quantification of complementary parallel and antiparallel hybrids of DNAs with pyrrolidine-based oxypeptide nucleic acids (POPNAs). When BODIPY-modified DNAs as fluorescent probe formed hybrids with complementary POPNAs, fluorescence of the BODIPY was effectively quenched by the guanine unit and the Lys unit on the POPNAs. The orientations of hybrids of POPNA with DNA were estimated by the quenching efficiencies of two BODIPY-modified DNAs. As a result, we clarified that configurations of POPNAs affect the extent of orientation of the hybrid duplexes.

引用

页码：607 / 610

页数：4

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