Biodegradable Combinatorial Drug Loaded pH-Sensitive Liposomes for Enhanced Osteosarcoma Therapeutics

Osteosarcoma, malignancy which is usually seen in adolescents and small children, in an absence of suitable treatment may lead to fatalities. The current available therapeutic opportunities like multidrug chemotherapy, although effective, have lots of side effects. Chemotherapy drugs along with nanocarriers may prove productive. This study describes the co-encapsulation/ combination of two chemotherapy drugs (doxorubicin and ladirubicin) within a nanocarrier liposome for specific targetivity of malignant tissues. The preparation and characterization of the combinatorial drug loaded liposomes have been described in detail. The encapsulation efficiency of the combinatorial drugs has been found to be enhanced than individual drug loaded liposomes. The TEM images indicate that the encapsulation of combination of drugs do not lead to distortion of the liposomes. Moreover the sizes of the liposomes are small enough to escape the endosomes. Cytocompatibility/biocompatibility of the combinatorial drug loaded liposomes with normal cells is no significantly different from that of control samples. The anti-tumor studies reveal an extremely effective drug delivery nanocarrier which results in reducing the tumor lesions to half its original size. The studies reveal that the co-encapsulation of drugs in liposomes increases the effectiveness of the drug delivery to malignant tissue.