CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

被引：46

作者：

Javaid, Nasir ^{[1
]}

Choi, Sangdun ^{[1
,2
]}

机构：

[1] Ajou Univ, Dept Mol Sci & Technol, Suwon, South Korea

[2] Ajou Univ, S&K Therapeut, Campus Plaza, Suwon, South Korea

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2021年 / 9卷

基金：

新加坡国家研究基金会;

关键词：

CRISPR; cas system; classification; host DNA repair; genome-editing efficiency; epigenome; HOMOLOGY-DIRECTED REPAIR; EMBRYONIC STEM-CELLS; GENOME-WIDE ANALYSIS; STRAND BREAK REPAIR; IN-VIVO; TARGETED MUTAGENESIS; GENE-EXPRESSION; GUIDE RNA; CRISPR-CAS9; NUCLEASES; CRYSTAL-STRUCTURE;

D O I：

10.3389/fcell.2021.761709

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications.

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页数：25

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