CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

被引：44
作者：
Javaid, Nasir [1 ]
Choi, Sangdun [1 ,2 ]
机构：
[1] Ajou Univ, Dept Mol Sci & Technol, Suwon, South Korea
[2] Ajou Univ, S&K Therapeut, Campus Plaza, Suwon, South Korea
来源：
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2021年 / 9卷
基金：
新加坡国家研究基金会;
关键词：
CRISPR; cas system; classification; host DNA repair; genome-editing efficiency; epigenome; HOMOLOGY-DIRECTED REPAIR; EMBRYONIC STEM-CELLS; GENOME-WIDE ANALYSIS; STRAND BREAK REPAIR; IN-VIVO; TARGETED MUTAGENESIS; GENE-EXPRESSION; GUIDE RNA; CRISPR-CAS9; NUCLEASES; CRYSTAL-STRUCTURE;
D O I：
10.3389/fcell.2021.761709
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications.
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页数：25
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