Choreography of bacteriophage T7 DNA replication

被引:50
作者
Lee, Seung-Joo [1 ]
Richardson, Charles C. [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
基金
美国国家卫生研究院;
关键词
ESCHERICHIA-COLI THIOREDOXIN; LAGGING-STRAND SYNTHESIS; GENE-4; HELICASE-PRIMASE; SSDNA-BINDING-PROTEIN; PROCESSIVITY FACTOR; SINGLE-MOLECULE; POLYMERASE-ACTIVITY; CRYSTAL-STRUCTURE; RING HELICASE; DUPLEX DNA;
D O I
10.1016/j.cbpa.2011.07.024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The replication system of phage T7 provides a model for DNA replication. Biochemical, structural, and single-molecule analyses together provide insight into replisome mechanics. A complex of polymerase, a processivity factor, and helicase mediates leading strand synthesis. Establishment of the complex requires an interaction of the C-terminal tail of the helicase with the polymerase. During synthesis the complex is stabilized by other interactions to provide for a processivity of 5 kilobase (kb). The C-terminal tail also interacts with a distinct region of the polymerase to captures dissociating polymerase to increase the processivity to >17 kb. The lagging strand is synthesized discontinuously within a loop that forms and resolves during each cycle of Okazaki fragment synthesis. The synthesis of a primer as well as the termination of a fragment signal loop resolution.
引用
收藏
页码:580 / 586
页数:7
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