共 50 条
Choreography of bacteriophage T7 DNA replication
被引:50
作者:
Lee, Seung-Joo
[1
]
Richardson, Charles C.
[1
]
机构:
[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
基金:
美国国家卫生研究院;
关键词:
ESCHERICHIA-COLI THIOREDOXIN;
LAGGING-STRAND SYNTHESIS;
GENE-4;
HELICASE-PRIMASE;
SSDNA-BINDING-PROTEIN;
PROCESSIVITY FACTOR;
SINGLE-MOLECULE;
POLYMERASE-ACTIVITY;
CRYSTAL-STRUCTURE;
RING HELICASE;
DUPLEX DNA;
D O I:
10.1016/j.cbpa.2011.07.024
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The replication system of phage T7 provides a model for DNA replication. Biochemical, structural, and single-molecule analyses together provide insight into replisome mechanics. A complex of polymerase, a processivity factor, and helicase mediates leading strand synthesis. Establishment of the complex requires an interaction of the C-terminal tail of the helicase with the polymerase. During synthesis the complex is stabilized by other interactions to provide for a processivity of 5 kilobase (kb). The C-terminal tail also interacts with a distinct region of the polymerase to captures dissociating polymerase to increase the processivity to >17 kb. The lagging strand is synthesized discontinuously within a loop that forms and resolves during each cycle of Okazaki fragment synthesis. The synthesis of a primer as well as the termination of a fragment signal loop resolution.
引用
收藏
页码:580 / 586
页数:7
相关论文