Oriented Immobilization of Enzymes Made Fit for Applied Biocatalysis: Non-Covalent Attachment to Anionic Supports using Zbasic2 Module

被引:44
作者
Wiesbauer, Johanna [1 ]
Bolivar, Juan M. [1 ]
Mueller, Mario [1 ]
Schiller, Margaretha [1 ]
Nidetzky, Bernd [1 ,2 ]
机构
[1] Graz Univ Technol, Inst Biotechnol & Biochem Engn, A-8010 Graz, Austria
[2] Austrian Res Ctr Ind Biotechnol ACIB GmbH, A-8010 Graz, Austria
基金
奥地利科学基金会;
关键词
supported catalyst; biocatalysis; ion exchange; proteins; enzymes; AMINO-ACID OXIDASE; SITE-SPECIFIC IMMOBILIZATION; PENICILLIN-G ACYLASE; DIRECTED MUTAGENESIS; SELECTIVE ADSORPTION; ESCHERICHIA-COLI; FUSION PROTEINS; PURIFICATION; STABILIZATION; POLYARGININE;
D O I
10.1002/cctc.201100103
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
[No abstract available]
引用
收藏
页码:1299 / 1303
页数:5
相关论文
共 52 条
[1]   Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst [J].
Abad, Sandra ;
Nahalka, Jozef ;
Bergler, Gabriele ;
Arnold, S. Alison ;
Speight, Robert ;
Fotheringham, Ian ;
Nidetzky, Bernd ;
Glieder, Anton .
MICROBIAL CELL FACTORIES, 2010, 9
[2]   Stabilization of penicillin G acylase from Escherichia coli:: Site-directed mutagenesis of the protein surface to increase multipoint covalent attachment [J].
Abian, O ;
Grazú, V ;
Hermoso, J ;
González, R ;
García, JL ;
Fernández-Lafuente, R ;
Guisán, JM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (02) :1249-1251
[3]   Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports [J].
Armisén, P ;
Mateo, C ;
Cortés, E ;
Barredo, JL ;
Salto, F ;
Diez, B ;
Rodés, L ;
García, JL ;
Fernández-Lafuente, R ;
Guisán, JM .
JOURNAL OF CHROMATOGRAPHY A, 1999, 848 (1-2) :61-70
[4]   Site-specific immobilization of genetically engineered variants of Candida antarctica lipase B [J].
Blank, Kerstin ;
Morfill, Julia ;
Gaub, Hermann E. .
CHEMBIOCHEM, 2006, 7 (09) :1349-1351
[5]  
Bornscheuer U.T., 2003, Angewandte Chemie, V115, P3458
[6]   Immobilizing enzymes: How to create more suitable biocatalysts [J].
Bornscheuer, UT .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (29) :3336-3337
[7]  
BUCHHOLZ K, 2005, BIOCATALYSIS ENZYME, P244
[8]  
Cao L, 2005, CARRIER-BOUND IMMOBILIZED ENZYMES: PRINCIPLES, APPLICATIONS AND DESIGN, P1, DOI 10.1002/3527607668
[9]   Cross-linked enzyme aggregates: A simple and effective method for the immobilization of penicillin acylase [J].
Cao, LQ ;
van Rantwijk, F ;
Sheldon, RA .
ORGANIC LETTERS, 2000, 2 (10) :1361-1364
[10]   One-step enzyme extraction and immobilization for biocatalysis applications [J].
Cassimjee, Karim Engelmark ;
Kourist, Robert ;
Lindberg, Diana ;
Larsen, Marianne Wittrup ;
Nguyen Hong Thanh ;
Widersten, Mikael ;
Bornscheuer, Uwe T. ;
Berglund, Per .
BIOTECHNOLOGY JOURNAL, 2011, 6 (04) :463-469