Phylogenetic evidence for the early evolution of microcystin synthesis

被引:396
作者
Rantala, A
Fewer, DP
Hisbergues, M
Rouhiainen, L
Vaitomaa, J
Börner, T
Sivonen, K
机构
[1] Univ Helsinki, Dept Appl Chem & Microbiol, Viikki Bioctr, FIN-00014 Helsinki, Finland
[2] Humboldt Univ, Inst Biol Genet, D-10115 Berlin, Germany
[3] Inst Pasteur, Inst Biol, F-59019 Lille, France
关键词
D O I
10.1073/pnas.0304489101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cyanobacteria are a prolific source of secondary metabolites, including compounds with toxic and enzyme-inhibiting activities. Microcystins and nodularins are the end products of a secondary metabolic pathway comprised of mixed polyketide synthases and nonribosomal peptide synthetases. Both peptides are potent natural toxins produced by distantly related genera of cyanobacteria. Horizontal gene transfer is thought to play a role in the sporadic distribution of microcystin producers among cyanobacteria. Our phylogenetic analyses indicate a coevolution of housekeeping genes and microcystin synthetase genes for the entire evolutionary history of the toxin. Hence they do not corroborate horizontal transfer of genes for microcystin biosynthesis between the genera. The sporadic distribution of microcystin synthetase genes in modern cyanobacteria suggests that the ability to produce the toxin has been lost repeatedly in the more derived lineages of cyanobacteria. The data we present here strongly suggest that the genes encoding nodularin synthetase are recently derived from those encoding microcystin synthetase.
引用
收藏
页码:568 / 573
页数:6
相关论文
共 58 条
[1]   Microfossils in 2000 Ma old cherty stromatolites of the Franceville Group, Gabon [J].
Amard, B ;
BertrandSarfati, J .
PRECAMBRIAN RESEARCH, 1997, 81 (3-4) :197-221
[2]  
[Anonymous], 1998, HLTH CRIT OTH SUPP I
[3]   Three dehydrobutyrine-containing microcystins from Nostoc [J].
Beattie, KA ;
Kaya, K ;
Sano, T ;
Codd, GA .
PHYTOCHEMISTRY, 1998, 47 (07) :1289-1292
[4]   Microcystin biosynthesis in Planktothrix:: Genes, evolution, and manipulation [J].
Christiansen, G ;
Fastner, J ;
Erhard, M ;
Börner, T ;
Dittmann, E .
JOURNAL OF BACTERIOLOGY, 2003, 185 (02) :564-572
[5]   Nonribosomal peptide synthetase genes occur in most cyanobacterial genera as evidenced by their distribution in axenic strains of the PCC [J].
Christiansen, G ;
Dittmann, E ;
Ordorika, LV ;
Rippka, R ;
Herdman, M ;
Börner, T .
ARCHIVES OF MICROBIOLOGY, 2001, 176 (06) :452-458
[6]   FORAGING ON CYANOBACTERIA BY COPEPODS - RESPONSES TO CHEMICAL DEFENSES AND RESOURCE ABUNDANCE [J].
DEMOTT, WR ;
MOXTER, F .
ECOLOGY, 1991, 72 (05) :1820-1834
[7]   Altered expression of two light-dependent genes in a microcystin-lacking mutant of Microcystis aeruginosa PCC 7806 [J].
Dittmann, E ;
Erhard, M ;
Kaebernick, M ;
Scheler, C ;
Neilan, BA ;
von Döhren, H ;
Börner, T .
MICROBIOLOGY-SGM, 2001, 147 :3113-3119
[8]   Insertional mutagenesis of a peptide synthetase gene that is responsible for hepatotoxin production in the cyanobacterium Microcystis aeruginosa PCC 7806 [J].
Dittmann, E ;
Neilan, BA ;
Erhard, M ;
vonDohren, H ;
Borner, T .
MOLECULAR MICROBIOLOGY, 1997, 26 (04) :779-787
[9]   The biosynthetic gene cluster for the antitumor drug bleomycin from Streptomyces verticillus ATCC15003 supporting functional interactions between nonribosomal peptide synthetases and a polyketide synthase [J].
Du, LC ;
Sánchez, C ;
Chen, M ;
Edwards, DJ ;
Shen, B .
CHEMISTRY & BIOLOGY, 2000, 7 (08) :623-642
[10]   Hybrid peptide-polyketide natural products:: Biosynthesis and prospects toward engineering novel molecules [J].
Du, LH ;
Sánchez, C ;
Shen, B .
METABOLIC ENGINEERING, 2001, 3 (01) :78-95