Peptide reactivity between multiple sclerosis (MS) CSF IgG and recombinant antibodies generated from clonally expanded plasma cells in MS CSF

被引：16

作者：

Yu, Xiaoli ^{[1
]}

Gilden, Don ^{[1
,2
]}

Schambers, Laura ^{[1
]}

Barmina, Olga ^{[1
]}

Burgoon, Mark ^{[1
]}

Bennett, Jeffrey ^{[1
,3
]}

Owens, Greg ^{[1
]}

机构：

[1] Univ Colorado Denver, Dept Neurol, Aurora, CO 80045 USA

[2] Univ Colorado Denver, Dept Microbiol, Aurora, CO 80045 USA

[3] Univ Colorado Denver, Dept Ophthalmol, Aurora, CO 80045 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2011年 / 233卷 / 1-2期

关键词：

Multiple sclerosis; Random peptide library; Epitope; Mimotope;

D O I：

10.1016/j.jneuroim.2010.11.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We employed 19 recombinant antibodies (rAbs) generated from clonally expanded plasma cells, and native IgG from cerebrospinal fluid (CSF) of three multiple sclerosis (MS) patients for panning with phage displayed random peptide libraries. Specific peptide epitopes/mimotopes were identified and characterized. Importantly, peptide-antibody interactions were shared by rAbs and native IgG from the same patient. Three peptides strongly interacted with at least one other MS CSF, but not to inflammatory CNS controls. Database searches revealed several protein candidates including stress proteins, cell surface proteins, and neuronal proteins. Peptides derived from the candidate proteins were recognized by rAbs. Identification of peptide epitopes/mimotopes in MS may provide clues regarding disease-relevant antigens. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：192 / 203

页数：12